Rhot1 Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human small intestine shows positivity in glandular cells.
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human cerebellum shows positivity in neuronal cells.
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human cerebral cortex shows positivity in neuronal cells.
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human liver shows positivity in hepatocytes.
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human pancreas shows positivity in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

Rhot1 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: THIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIE
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RHOT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Reactivity reported in (PMID: 26259702)
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot Reactivity reported in (PMID:24671417)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Rhot1 Protein (NBP1-89011PEP)
Publications
Read Publications using
NBP1-89011 in the following applications:

Reactivity Notes

Rat reactivity reported in (PMID: 26631479).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rhot1 Antibody - BSA Free

  • ARHT1
  • EC 3.6.5
  • EC 3.6.5.-
  • FLJ11040
  • FLJ12633
  • hMiro-1
  • Miro1
  • MIRO-1mitochondrial Rho GTPase 1
  • mitochondrial Rho 1
  • rac-GTP binding protein-like protein
  • Rac-GTP-binding protein-like protein
  • Ras homolog gene family member T1
  • ras homolog gene family, member T1
  • Rhot1

Background

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rhot1 Antibody (NBP1-89011)(9)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, WB, Western Blot.


Filter By Application
ICC/IF
(3)
WB
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-89011 Applications Species
Hanna Lee, Ok-Yi Jeong, Hee Jin Park, Sung-Lim Lee, Eun-yeong Bok, Mingyo Kim, Young Sun Suh, Yun-Hong Cheon, Hyun-Ok Kim, Suhee Kim, Sung Hak Chun, Jung Min Park, Young Jin Lee, Sang-Il Lee Promising Therapeutic Effects of Embryonic Stem Cells-Origin Mesenchymal Stem Cells in Experimental Pulmonary Fibrosis Models: Immunomodulatory and Anti-Apoptotic Mechanisms Immune Network 2023-12-01 [PMID: 38188598]
Fagbadebo F, Kaiser P, Zittlau K et al. A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1 Frontiers in Molecular Biosciences 2022-03-10 [PMID: 35359597] (ICC/IF, Human) ICC/IF Human
Furnish M, Boulton DP, Genther V et al. MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent Stress Signaling Molecular Cancer Research 2022-04-01 [PMID: 34992146] (Western Blot) Western Blot
Stephen TL, Higgs NF, Sheehan DF et al. Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling. J Neurosci 2015-12-02 [PMID: 26631479] (WB, Rat) WB Rat
Kanfer G, Thibault Courtheoux T, Martin Peterka M et al. Mitotic redistribution of the mitochondrial network by Miro and Cenp-F. Nat Commun 2015-08-11 [PMID: 26259702] (ICC/IF, Human) ICC/IF Human
Bingol B, Tea JS, Phu L et al. The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy. Nature 2014-06-19 [PMID: 24896179]
Birsa N, Norkett R, Wauer T et al. Lysine 27 Ubiquitination of the Mitochondrial Transport Protein Miro Is Dependent on Serine 65 of the Parkin Ubiquitin Ligase. J Biol Chem 2014-05-23 [PMID: 24671417] (WB, ICC/IF, Human) WB, ICC/IF Human
Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]
De Vos KJ, Morotz GM, Stoica R et al. VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis. Hum Mol Genet 2012-03-01 [PMID: 22131369]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rhot1 Antibody (NBP1-89011) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RHOT1