![Western Blot: Kinesin 5B Antibody [NBP2-21667] - Western Blot of Kinesin 5B antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:40,000 for 1hr at RT.Block: 5% BLOTTO 1 hr at RT.Predicted/Observed size: 72 kDa, ~70 kDa for Kinesin 5B. Other band(s): degradation.](http://images.novusbio.com/fullsize/Kinesin-5B-Antibody-Western-Blot-NBP2-21667-img0006.jpg "Western Blot: Kinesin 5B Antibody [NBP2-21667] - Western Blot of Kinesin 5B antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:40,000 for 1hr at RT.Block: 5% BLOTTO 1 hr at RT.Predicted/Observed size: 72 kDa, ~70 kDa for Kinesin 5B. Other band(s): degradation.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, PAGE |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | The product was prepared from monospecific antiserum by delipidation and defibrination Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Kinesin 5B Antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human Kinesin 5B protein. (Uniprot: P33176) |
| Isotype | Serum |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KIF5B |
| Purity | Delipidation and Defibrination |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested for use in SDS, western blot, and immunohistochemistry. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user. Use in ICC/IF was reported in scientific literature (PMID: 30429451). |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Delipidation and Defibrination |
| Publication using NBP2-21667 | Applications | Species |
|---|---|---|
| Choi GE, Oh JY, Lee HJ et al. Glucocorticoid-mediated ER-mitochondria contacts reduce AMPA receptor and mitochondria trafficking into cell terminus via microtubule destabilization Cell Death Dis. 2018-11-13 [PMID: 30429451] (ICC/IF, WB, Human) | ICC/IF, WB | Human |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - Immunohistochemistry of Kinesin 5B antibody. Tissue: Human Brain at 40X at pH 6.](http://images.novusbio.com/fullsize/Kinesin 5B Antibody-Immunohistochemistry-NBP2-21667-img0008.jpg "Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - Immunohistochemistry of Kinesin 5B antibody. Tissue: Human Brain at 40X at pH 6.")
![Western Blot: Kinesin 5B Antibody [NBP2-21667] - Glucocorticoid promoted microtubule destabilization and following transport impairment. The hippocampus of mice exposed to rapamycin (8 mg/kg) for 2 days before corticosterone (10 mg/kg) for 24 h was collected and lysed. alpha-tubulin was co-immunoprecipitated with an anti-kinesin-1 antibody (the left side). Expression of kinesin-1 and alpha-tubulin in total cell lysates is shown in the right side. ** indicates p < 0.01 vs. vehicle. # indicates p < 0.05 vs. corticosterone alone. n = 4. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1172-y) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Kinesin-5B-Antibody-Western-Blot-NBP2-21667-img0004.jpg "Western Blot: Kinesin 5B Antibody [NBP2-21667] - Glucocorticoid promoted microtubule destabilization and following transport impairment. The hippocampus of mice exposed to rapamycin (8 mg/kg) for 2 days before corticosterone (10 mg/kg) for 24 h was collected and lysed. alpha-tubulin was co-immunoprecipitated with an anti-kinesin-1 antibody (the left side). Expression of kinesin-1 and alpha-tubulin in total cell lysates is shown in the right side. ** indicates p < 0.01 vs. vehicle. # indicates p < 0.05 vs. corticosterone alone. n = 4. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-1172-y) licensed under a CC-BY license.")
![Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - 40X in human brain at pH 6. Tissue: Human Brain.](http://images.novusbio.com/fullsize/Kinesin-5B-Antibody-Immunohistochemistry-NBP2-21667-img0003.jpg "Immunohistochemistry: Kinesin 5B Antibody [NBP2-21667] - 40X in human brain at pH 6. Tissue: Human Brain.")
![SDS-Page: Kinesin 5B Antibody [NBP2-21667] - SDS-PAGE of Kinesin 5B antibody. Lane 1: Truncated Kinesin-1 Protein. Lane 2: none.Load: 5 ug per lane.Block: coomassie blue for 20 min, destain overnight at RT.Predicted/Observed size: 72 kDa, 72kDa for Kinesin 5B. Other band(s): degradation.](http://images.novusbio.com/fullsize/Kinesin-5B-Antibody-SDS-Page-NBP2-21667-img0007.jpg "SDS-Page: Kinesin 5B Antibody [NBP2-21667] - SDS-PAGE of Kinesin 5B antibody. Lane 1: Truncated Kinesin-1 Protein. Lane 2: none.Load: 5 ug per lane.Block: coomassie blue for 20 min, destain overnight at RT.Predicted/Observed size: 72 kDa, 72kDa for Kinesin 5B. Other band(s): degradation.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")