JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 3 applications: IHC, Immunohistochemistry, Western Blot.

Showing Publications 1 - 10 of 25. Show All 25 Publications. Collapse Publications.

Publications using AF1205	Applications	Species
Geou-Yarh Liou, Alicia K. Fleming Martinez, Heike R. Döppler, Ligia I. Bastea, Peter Storz Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth iScience 2023-06-16 [PMID: 37250781] (Immunohistochemistry, Western Blot, Transgenic Mouse)	Immunohistochemistry, Western Blot	Transgenic Mouse
Geou-Yarh Liou, Alicia K. Fleming Martinez, Heike R. Döppler, Ligia I. Bastea, Peter Storz Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth iScience 6/16/2023 [PMID: 37250781]
K Suyama, D Sakai, S Hayashi, N Qu, H Terayama, D Kiyoshima, K Nagahori, M Watanabe Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70 Biomedicines, 2023-03-12;11(3):. 2023-03-12 [PMID: 36979842] (Western Blot, Human)	Western Blot	Human
Ting Niu, Zhiying Wei, Jiao Fu, Shu Chen, Ru Wang, Yuya Wang et al. Venlafaxine, an anti-depressant drug, induces apoptosis in MV3 human melanoma cells through JNK1/2-Nur77 signaling pathway Frontiers in Pharmacology 1/4/2023 [PMID: 36686679]
T Ishijima, K Nakajima Changes of signaling molecules in the axotomized rat facial nucleus Journal of chemical neuroanatomy, 2022-10-29;126(0):102179. 2022-10-29 [PMID: 36341893] (IHC, Rat)	IHC	Rat
Emilia Alors-Perez, Ricardo Blázquez-Encinas, Sonia Alcalá, Cristina Viyuela-García, Sergio Pedraza-Arevalo, Vicente Herrero-Aguayo et al. Dysregulated splicing factor SF3B1 unveils a dual therapeutic vulnerability to target pancreatic cancer cells and cancer stem cells with an anti-splicing drug Journal of Experimental & Clinical Cancer Research 12/2/2021 [PMID: 34857016]
S Dey, LM Udari, P RiveraHern, JJ Kwon, B Willis, JJ Easler, EL Fogel, S Pandol, J Kota Loss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis JCI Insight, 2021-10-08;0(0):. 10/8/2021 [PMID: 34464354]
EM Mantawy, RS Said, DH Kassem, AK Abdel-Aziz, AM Badr Novel molecular mechanisms underlying the ameliorative effect of N-acetyl-L-cysteine against ?-radiation-induced premature ovarian failure in rats Ecotoxicol. Environ. Saf., 2020-08-29;206(0):111190. 2020-08-29 [PMID: 32871518] (IHC, Rat)	IHC	Rat
J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020-05-04;15(5):e0232779. 2020-05-04 [PMID: 32365083] (Western Blot, Mouse)	Western Blot	Mouse
JM Jiménez-Va, V Herrero-Ag, AJ Montero-Hi, E Gómez-Góme, AC Fuentes-Fa, AJ León-Gonzá, P Sáez-Martí, E Alors-Pére, S Pedraza-Ar, T González-S, O Reyes, A Martínez-L, R Sánchez-Sá, S Ventura, EM Yubero-Ser, MJ Requena-Ta, JP Castaño, MD Gahete, RM Luque Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer EBioMedicine, 2020-01-03;51(0):102547. 2020-01-03 [PMID: 31902674] (Western Blot, Human)	Western Blot	Human
Chen HC, Chen MY, Hsieh CL et al. TRPV1 is a Responding Channel for Acupuncture Manipulation in Mice Peripheral and Central Nerve System. Cell. Physiol. Biochem. 9/19/2018 [PMID: 30231245]
K Suyama, D Sakai, N Hirayama, Y Nakamura, E Matsushita, H Terayama, N Qu, O Tanaka, K Sakabe, M Watanabe Effects of interleukin-17A in nucleus pulposus cells and its small-molecule inhibitors for intervertebral disc disease J. Cell. Mol. Med., 2018-09-11;0(0):. 2018-09-11 [PMID: 30207057] (Western Blot, Rat)	Western Blot	Rat
S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 2017-07-27;8(7):e2955. 2017-07-27 [PMID: 28749468] (Western Blot, Human)	Western Blot	Human
EM Mantawy, A Esmat, WM El-Bakly, RA Salah ElDi, E El-Demerda Mechanistic clues to the protective effect of chrysin against doxorubicin-induced cardiomyopathy: Plausible roles of p53, MAPK and AKT pathways Sci Rep, 2017-07-06;7(1):4795. 2017-07-06 [PMID: 28684738] (Western Blot, Rat)	Western Blot	Rat
Pregnane X receptor activation attenuates inflammation-associated intestinal epithelial barrier dysfunction by inhibiting cytokine-induced MLCK expression and JNK1/2 activation J Pharmacol Exp Ther, 2016-07-20;0(0):. 7/20/2016 [PMID: 27440420]
Sana Khalid, Astrid Drasche, Marco Thurner, Martin Hermann, Muhammad Imtiaz Ashraf, Friedrich Fresser et al. cJun N-terminal kinase (JNK) phosphorylation of serine 36 is critical for p66Shc activation Scientific Reports 2/12/2016 [PMID: 26868434]
Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Nat Cell Biol, 2014-12-08;17(1):57-67. 2014-12-08 [PMID: 25487280] (Western Blot, Human)	Western Blot	Human
Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. PLoS ONE, 2014-07-28;9(7):e103148. 2014-07-28 [PMID: 25068294] (Western Blot, Mouse)	Western Blot	Mouse
Ashraf M, Ebner M, Wallner C, Haller M, Khalid S, Schwelberger H, Koziel K, Enthammer M, Hermann M, Sickinger S, Soleiman A, Steger C, Vallant S, Sucher R, Brandacher G, Santer P, Dragun D, Troppmair J A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury. Cell Commun Signal, 2014-01-14;12(0):6. 2014-01-14 [PMID: 24423080] (Western Blot, Rat)	Western Blot	Rat
Katarzyna Koziel, Julija Smigelskaite, Astrid Drasche, Marion Enthammer, Muhammad Imtiaz Ashraf, Sana Khalid et al. RAF and antioxidants prevent cell death induction after growth factor abrogation through regulation of Bcl-2 proteins☆ Experimental Cell Research 10/15/2013 [PMID: 23933517]
Justyna Sosna, Susann Voigt, Sabine Mathieu, Arne Lange, Lutz Thon, Parvin Davarnia et al. TNF-induced necroptosis and PARP-1-mediated necrosis represent distinct routes to programmed necrotic cell death Cellular and Molecular Life Sciences 1/1/2014 [PMID: 23760205]
Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. J Biol Chem, 2012-06-15;287(32):26478-94. 2012-06-15 [PMID: 22707717] (Western Blot, Human)	Western Blot	Human
Protective effect of the poly(ADP-ribose) polymerase inhibitor PJ34 on mitochondrial depolarization-mediated cell death in hepatocellular carcinoma cells involves attenuation of c-Jun N-terminal kinase-2 and protein kinase B/Akt activation. Mol. Cancer, 2012-05-14;11(0):34. 2012-05-14 [PMID: 22583868] (Western Blot, Human)	Western Blot	Human
Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400. 2009-06-12 [PMID: 19525388] (Western Blot, Human)	Western Blot	Human
Bengochea A, de Souza MM, Lefrancois L, Le Roux E, Galy O, Chemin I, Kim M, Wands JR, Trepo C, Hainaut P, Scoazec JY, Vitvitski L, Merle P Common dysregulation of Wnt/Frizzled receptor elements in human hepatocellular carcinoma. Br. J. Cancer, 2008-06-24;99(1):143-50. 2008-06-24 [PMID: 18577996] (Western Blot, Human)	Western Blot	Human
Show All 25 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	3 reviewed by: Aleksandra Hac	WB	Human and Mouse	05/04/2017	View Summary Application Western Blot Sample Tested HDFa whole cell lysate,MEF whole cell lysate,PC3 Whole Cell Lysate (Human prostate cancer cell) Species Human and Mouse Lot HCT0517011 Comments Comments We obtained bands at predicted molecular weight for both human (PC3, HDFa) as well as mouse (MEF) samples. However, at recommended concentrations the signal was quite weak using ECL substrate. Perhaps they work better using substrates with higher sensitivity.

Array AF1205

• JNK1/2/3 Antibodies
• JNK1/2/3 ELISA