Recombinant Mouse CD7 Protein, CF Summary
Details of Functionality |
Measured by its binding ability in a functional ELISA. When Recombinant Mouse SECTM1 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CD7 that produces 50% optimal binding response is approximately 15‑75 ng/mL. |
Source |
Mouse myeloma cell line, NS0-derived mouse CD7 protein Gln24-Pro150, with a C-terminal 6-His tag |
Accession # |
|
N-terminal Sequence |
No results obtained: Gln24 predicted |
Protein/Peptide Type |
Recombinant Proteins |
Gene |
Cd7 |
Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
20-35 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions |
Reconstitute at 100 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse CD7 Protein, CF
Background
CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily (1). Mature mouse CD7 consists of a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 39 aa cytoplasmic domain (2). Within the ECD, mouse CD7 shares 43% and 81% aa sequence identity with human and rat CD7, respectively. CD7 is expressed on T cells (2, 3), NK cells (4), myeloid progenitor cells (5), and CD19
+ B progenitor cells (6). Among CD8
+ T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells (7). CD7 associates
in cis with CD3 and CD45 and
in trans with SECTM1 (8, 9). Ligation of CD7 with antibodies or SECTM1 co-stimulates the activation and proliferation of CD4
+ and CD8
+ T cells (10, 11) and NK cells (4, 9). It also enhances integrin-mediated adhesion of these cells to Fibronectin, ICAM-1, and VCAM-1 (3, 12). CD7 additionally mediates the Galectin-1 induced apoptosis of T cells (13) and facilitates the fusion of HIV-1 with target cells (14).
- Sutherland, D.R. et al. (1984) J. Immunol. 133:327.
- Yoshikawa, K. et al. (1993) Immunogenetics 37:114.
- Shimizu, Y. et al. (1992) J. Exp. Med. 175:577.
- Rabinowich, H. et al. (1994) J. Immunol. 152:517.
- Chabannon, C. et al. (1992) J. Immunol. 149:2110.
- Grumayer, E.R. et al. (1991) Blood 77:64.
- Aandahl, E.M. et al. (2003) J. Immunol. 170:2349.
- Lazarovits, A.I. et al. (1994) J. Immunol. 153:3956.
- Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
- Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
- Carrera, A.C. et al. (1988) J. Immunol. 141:1919.
- Rabinowich, H. et al. (1994) J. Immunol. 153:3504.
- Pace, K.E. et al. (2000) J. Immunol. 165:2331.
- Sato, A.I. et al. (1994) J. Immunol. 152:5142.
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