Recombinant Human Integrin alpha 4 beta 1 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Integrin alpha 4 beta 1, also called VLA4, is an integrin family adhesion receptor that shares the beta 1 subunit with eleven other family members and the alpha 4 subunit with integrin alpha 4 beta 7 (1 - 4). The non-covalent heterodimer of 150 kDa alpha 4/CD49d and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits mediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragment of fibronectin in the extracellular matrix (2 - 4). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 4 beta -propeller to form a binding domain when the dimer is in active, extended and open conformation. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 944 aa human alpha 4 extracellular domain (ECD) shares 85% aa identity with mouse, rat and canine alpha 4, while the 708 aa human beta 1 ECD shares 92 - 96% aa identity with rat, bovine, mouse, and feline beta 1. Five alternate splice forms of the human beta 1 cytoplasmic domain, including one antagonistic form, vary by 12 to 48 aa and show differential expression patterns (5). Leukocytes (except for neutrophils), erythroid precursors and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express alpha 4 beta 1 (6 - 10). Deletion is lethal in the mouse embryo due to faulty placentation and development of the epicardium and coronary vessels (7, 10). In the adult, alpha 4 beta 1 primarily regulates immune cell migration (11 - 13). Circulating leukocyte alpha 4 beta 1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as SDF-1 (11). This activation facilitates rolling, firm adhesion, and extravasation. Interfering with leukocyte migration via the therapeutic alpha 4 beta 1antibody Natalizumab can reduce the severity of autoimmune disorders such as multiple sclerosis (12). Natalizumab can also mobilize hematopoietic precursors from the bone marrow by impeding their interaction with stromal cell VCAM-1 (8, 12). During immune cell activation, alpha 4 beta 1 can function as a costimulatory molecule (13, 14).

We have publications tested in 2 confirmed species: Human, Equine.

We have publications tested in 4 applications: Bioassay, ELISA Capture, SPR, Surface Plasmon Resonance.

Showing Publications 1 - 7 of 7.

Publications using 5668-A4	Applications	Species
Naoi, Y;Morinaga, T;Nagasaki, J;Ariyasu, R;Ueda, Y;Yamashita, K;Zhou, W;Kawashima, S;Kawase, K;Honobe-Tabuchi, A;Ohnuma, T;Kawamura, T;Umeda, Y;Kawahara, Y;Nakamura, Y;Kiniwa, Y;Yamasaki, O;Fukushima, S;Kawazu, M;Suzuki, Y;Nishikawa, H;Hanazawa, T;Ando, M;Inozume, T;Togashi, Y; CD106 in tumor-specific exhausted CD8+ T cells mediates immunosuppression by inhibiting TCR signaling Cancer research 2024-04-18 [PMID: 38635899] (Bioassay, Human)	Bioassay	Human
C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family PLoS ONE, 2022-09-15;17(9):e0274667. 2022-09-15 [PMID: 36108060] (ELISA Capture, Human)	ELISA Capture	Human
G Fnu, P Agrawal, GC Kundu, GF Weber Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44 Biomolecules, 2021-05-30;11(6):. 2021-05-30 [PMID: 34070790] (SPR, Human)	SPR	Human
S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7 PLoS Pathog., 2018-08-28;14(8):e1007278. 2018-08-28 [PMID: 30153309] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
EC Skoog, VA Morikis, ME Martin, GA Foster, LP Cai, LM Hansen, B Li, JA Gaddy, SI Simon, JV Solnick CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding MBio, 2018-05-15;9(3):. 2018-05-15 [PMID: 29764950] (Bioassay)	Bioassay
Chakraborty S, Hu S, Wu S, Karmenyan A, Chiou A The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET. PLoS ONE, 2015-03-20;10(3):e0121399. 2015-03-20 [PMID: 25793408] (Bioassay, Human)	Bioassay	Human
Azab W, Zajic L, Osterrieder N The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding. Vet Res, 2012-08-21;43(1):61. 2012-08-21 [PMID: 22909178] (Bioassay, Equine)	Bioassay	Equine

Array 5668-A4

• Integrin alpha 4 beta 1 Antibodies
• Integrin alpha 4 beta 1 Proteins and Enzymes
• Integrin alpha 4 beta 1 ELISA