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Recombinant Human IL-9R (NS0-expressed) Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
290-RNS-025/CF
Carrier Free
290-RNS-025/CF
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Recombinant Human IL-9R (NS0-expressed) Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-9-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 1-4 µg/mL in the presence of 2 ng/mL of recombinant human IL-9.
Source
Mouse myeloma cell line, NS0-derived human IL-9 R protein
Val40-Pro270, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Val40
Protein/Peptide Type
Recombinant Proteins
Gene
IL9R
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
27.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38 kDa, reducing conditions
Publications
Read Publication using
290-RNS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-9R (NS0-expressed) Protein

  • CD129 antigen
  • CD129
  • IL-9 R
  • IL-9 receptor
  • IL9R
  • IL-9R
  • interleukin 9 receptor
  • interleukin-9 receptor

Background

The specific high affinity functional IL-9 receptor complex consists of a ligand-specific IL-9 R( alpha ) subunit and the common gamma chain that is shared with the receptors for IL-2, IL-4, IL-7 and IL-15. Both the IL-9 R subunit and the common gamma chain are members of the hematopoietin receptor superfamily. The cDNAs encoding mouse and human IL-9 receptors have been isolated. The deduced mouse and human transmembrane proteins, sharing 53% amino acid sequence identity, contain 468 and 533 amino acid residues, respectively. As a result of alternative splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.

  1. Renauld, J. et al. (1992) Proc. Natl. Acad. Sci. USA, 89:5690.
  2. Kimura, Y. et al. (1995) Int. Immunol. 7:115.

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Publications for IL-9R (290-RNS)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol IL9R
Uniprot