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Recombinant Human CXCL6/GCP-2 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
333-GC-025/CF
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Recombinant Human CXCL6/GCP-2 Protein Summary

Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.5‑1.5 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 3‑15 ng/mL.
Source
E. coli-derived human CXCL6/GCP-2 protein
Val40-Asn114
Accession #
N-terminal Sequence
Val40
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL6
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
333-GC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL6/GCP-2 Protein

  • chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)
  • Chemokine alpha 3
  • CKA-3Granulocyte chemotactic protein 2
  • CXCL6
  • GCP-2
  • GCP2member 6 (granulocytechemotactic protein 2)
  • GCP-2Small-inducible cytokine B6
  • Small inducible cytokine subfamily B (Cys-X-Cys), member b

Background

GCP-2 (granulocyte chemotactic protein-2) also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level.

Human GCP-2 cDNA encodes a propeptide of 114 amino acid residues with a predicted 37 aa residue signal peptide and 77 aa residue mature protein. Several forms of natural GCP-2 have been isolated from MG-63 conditioned media, indicating that GCP-2 undergoes limited processing at both the N- and C-termini. Human GCP-2 is a primary response gene whose induction by cytokines is attenuated by dexamethasone.

Human GCP-2 and mouse GCP-2/LIX have been shown to chemoattract and activate neutrophils, but not eosinophils and monocytes. It is likely that GCP-2 activities are mediated via the human or mouse CXCR2.

  1. Proost, P. et al. (1993) J. Immunol. 150:1000.
  2. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  3. Rovai, L.E. et al. (1997) J. Immunol. 158:5257.
  4. Wuyts, A. et al. (1997) J. Immunol. 157:1736.

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Recombinant Human
CXCL6/GCP-2 Protein
333-GC
Recombinant Human CXCL6/GCP-2 Protein
Species: Hu
Applications: Bioactivity

Publications for CXCL6/GCP-2 (333-GC)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
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Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using 333-GC Applications Species
Shakiba, S;Haddadi, NS;Afshari, K;Lubov, JE;Raef, HS;Li, R;Yildiz-Altay, Ü;Daga, M;Refat, MA;Kim, E;de Laflin, JG;Akabane, A;Sherman, S;MacDonald, E;Strassner, JP;Zhang, L;Leon, M;Baer, CE;Dresser, K;Liang, Y;Whitley, JB;Skopelja-Gardner, S;Harris, JE;Deng, A;Vesely, MD;Rashighi, M;Richmond, J; Spatial characterization of interface dermatitis in cutaneous lupus reveals novel chemokine ligand-receptor pairs that drive disease bioRxiv : the preprint server for biology 2024-01-06 [PMID: 38260617] (Bioassay, Human) Bioassay Human
JC Ma, XW Sun, H Su, Q Chen, TK Guo, Y Li, XC Chen, J Guo, ZQ Gong, XD Zhao, JB Qi Fibroblast-derived CXCL12/SDF-1? promotes CXCL6 secretion and co-operatively enhances metastatic potential through the PI3K/Akt/mTOR pathway in colon cancer World J. Gastroenterol., 2017-07-28;23(28):5167-5178. 2017-07-28 [PMID: 28811711] (Bioassay, Human) Bioassay Human
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO J., 2012-07-31;31(17):3607-19. 2012-07-31 [PMID: 22850671] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Begley LA, Kasina S, Macdonald J, Macoska JA The inflammatory microenvironment of the aging prostate facilitates cellular proliferation and hypertrophy. Cytokine, 2008-06-24;43(2):194-9. 2008-06-24 [PMID: 18572414] (Bioassay, Human) Bioassay Human
Nguyen AN, Stebbins EG, Henson M, O&amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. Exp. Cell Res., 2006-04-04;312(10):1909-23. 2006-04-04 [PMID: 16600214] (Bioassay, Human) Bioassay Human

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Recombinant Human CXCL6/GCP‑2 (Catalog&nbsp;# 333-GC) induces chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog&nbsp;# AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP‑2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog&nbsp;# MAB333). The ND50 is typically 8-30&nbsp;μg/mL.
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Bioinformatics

Gene Symbol CXCL6
Uniprot