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CXCR1/IL-8RA Antibody (42705) [Unconjugated]

Images

 
Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more
CXCR1/IL-8 RA was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330) at 5 µg/mL overnight at 4 °C. ...read more
CXCR1/IL‑8 RA was detected in immersion fixed frozen sections of human lymph nodes using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more
Recombinant Human CXCL8/IL‑8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
42705
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CXCR1/IL-8RA Antibody (42705) [Unconjugated] Summary

Additional Information
Clone 42705 was used by HLDA to establish CD designation
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
Met1-Leu350
Accession # AAA59159
Specificity
Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CXCR1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.4-2.0 ug/mL
Publications
Read Publications using
MAB330 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR1/IL-8RA Antibody (42705) [Unconjugated]

  • CD128
  • CD181 antigen
  • CD181
  • CDw128aC-C
  • chemokine (C-X-C motif) receptor 1
  • CKR-1
  • CMKAR1
  • C-X-C chemokine receptor type 1
  • CXCR1
  • CXC-R1
  • CXCR-1
  • High affinity interleukin-8 receptor A
  • IL-8 RA
  • IL-8 receptor type 1
  • IL-8R A
  • IL8R1
  • IL8RA
  • IL8RAC-C-CKR-1
  • IL8RBA
  • interleukin 8 receptor, alpha
  • interleukin-8 receptor type 1
  • interleukin-8 receptor type A

Background

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR1/IL-8RA Antibody (MAB330)(50)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Guinea Pig, Rabbit.

We have publications tested in 10 applications: Bioassay, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, In Vivo, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(9)
Functional Assay
(1)
ICC
(3)
IHC
(2)
IHC-Fr
(3)
IHC-P
(2)
In Vivo
(1)
Neutralization
(20)
Western Blot
(3)
All Applications
Filter By Species
Human
(36)
Mouse
(3)
Rat
(1)
Guinea Pig
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using MAB330 Applications Species
Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y; IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer International immunopharmacology 2023-06-16 [PMID: 37331296] (Neutralization, Human) Neutralization Human
Tsukamoto, S;Koma, YI;Kitamura, Y;Tanigawa, K;Azumi, Y;Miyako, S;Urakami, S;Hosono, M;Kodama, T;Nishio, M;Shigeoka, M;Yokozaki, H; Matrix Metalloproteinase 9 Induced in Esophageal Squamous Cell Carcinoma Cells via Close Contact with Tumor-Associated Macrophages Contributes to Cancer Progression and Poor Prognosis Cancers 2023-05-30 [PMID: 37296952] (Western Blot, Human) Western Blot Human
Adrien Guérard, Victor Laurent, Gaëlle Fromont, David Estève, Julia Gilhodes, Edith Bonnelye et al. The Chemokine Receptor CCR3 Is Potentially Involved in the Homing of Prostate Cancer Cells to Bone: Implication of Bone-Marrow Adipocytes International Journal of Molecular Sciences 2021-02-17 [PMID: 33671469]
SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021-01-11;12(1):262. 2021-01-11 [PMID: 33431859] (IHC, Human) IHC Human
PC Hart, HA Kenny, N Grassl, KM Watters, LM Litchfield, F Coscia, I Blaženovi?, L Ploetzky, O Fiehn, M Mann, E Lengyel, IL Romero Mesothelial Cell HIF1alpha Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk Cell Rep, 2019-12-17;29(12):4086-4098.e6. 2019-12-17 [PMID: 31851935] (Neutralization, Mouse) Neutralization Mouse
AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming Cell Rep, 2019-11-19;29(8):2505-2519.e4. 2019-11-19 [PMID: 31747616] (Flow Cytometry, Human) Flow Cytometry Human
F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019-10-24;20(21):. 2019-10-24 [PMID: 31653015] (Neutralization, Human) Neutralization Human
Ofir Klein, Rebecca A. Krier-Burris, Pia Lazki-Hagenbach, Yaara Gorzalczany, Yang Mei, Peng Ji et al. Mammalian diaphanous-related formin 1 (mDia1) coordinates mast cell migration and secretion through its actin-nucleating activity Journal of Allergy and Clinical Immunology 2019-10-01 [PMID: 31279009]
Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 2019-04-30 [PMID: 31040288]
I Sharma, A Singh, F Siraj, S Saxena IL-8/CXCR1/2 signalling promotes tumor cell proliferation, invasion and vascular mimicry in glioblastoma J. Biomed. Sci., 2018-08-08;25(1):62. 2018-08-08 [PMID: 30086759] (Bioassay, Human) Bioassay Human
Show All 50 Publications.

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Bioinformatics

Gene Symbol CXCR1
Uniprot