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CXCL6/GCP-2 Antibody (60910)

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 (1 Review)
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Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP‑2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 μg/mL.
Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
60910
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB333-SP
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CXCL6/GCP-2 Antibody (60910) Summary

Immunogen
E. coli-derived recombinant human CXCL6/GCP-2
Val40-Asn114
Accession # P80162
Specificity
Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha , rhGRO beta , rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha , and rhSDF-1 beta is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCL6
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 8-30 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human CXCL6/GCP-2 Biotinylated Antibody (Catalog number BAF333)
Standard: Recombinant Human CXCL6/GCP-2 (Catalog number 333-GC)
Reviewed Applications
Read 1 Review rated 4
using
MAB333 in the following applications:
Publications
Read Publications using
MAB333 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL6/GCP-2 Antibody (60910)

  • chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)
  • Chemokine alpha 3
  • CKA-3Granulocyte chemotactic protein 2
  • CXCL6
  • GCP-2
  • GCP2member 6 (granulocytechemotactic protein 2)
  • GCP-2Small-inducible cytokine B6
  • Small inducible cytokine subfamily B (Cys-X-Cys), member b

Background

CXCL6, also known as Granulocyte Chemotactic Protein 2 (GCP-2), belongs to the CXC chemokine family. It functions as a neutrophil chemoattractant through interactions with CXCR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCL6/GCP-2
Antibody (60910)
MAB333
CXCL6/GCP-2 Antibody (60910)
Species: Hu
Applications: WB, B/N, ELISA(Cap)

Publications for CXCL6/GCP-2 Antibody (MAB333)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC-P, Neutralization, Western Blot.


Filter By Application
IHC-P
(1)
Neutralization
(1)
Western Blot
(1)
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Human
(2)
All Species

Review for CXCL6/GCP-2 Antibody (MAB333) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB333:
Filter by Applications
B/N
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Ruhul Choudhury
 B/N Mouse 01/20/2017
View

Summary

ApplicationBlock/Neutralize
Sample TestedUterine endothelial cells conditioned medium
SpeciesMouse

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
 
 Ruhul Choudhury
01/20/2017
Application: B/N
Species: Mouse
read this review

Bioinformatics

Gene Symbol CXCL6
Uniprot