![Western Blot: RAC3 Antibody (JE56-48) [NBP3-32888] - Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (NBP3-32888) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HUVEC cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (15 ug/Lane) Lane 6: C2C12 cell lysate (15 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32888) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32888_rabbit-rac3-mab-je56-48-288202412442984.jpg "Western Blot: RAC3 Antibody (JE56-48) [NBP3-32888] - Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (NBP3-32888) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: JAR cell lysate (20 ug/Lane) Lane 3: HUVEC cell lysate (20 ug/Lane) Lane 4: PC-12 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (15 ug/Lane) Lane 6: C2C12 cell lysate (15 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32888) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | JE56-48 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human RAC3 aa 71-170 / 192. (Uniprot: P60763) |
| Localization | Cytoplasm, Endomembrane system, Cell projection, lamellipodium, Cytoplasm, perinuclear region, Cell membrane, cytoskeleton. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RAC3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for RAC3 Antibody (NBP3-32888)Find related products by research area.
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| Gene Symbol | RAC3 |
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