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POLG2 Antibody

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Staining of human cell line U2OS shows localization to mitochondria.
Staining of human cell line U2OS shows localization to mitochondria.
Analysis in human cell line RT-4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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POLG2 Antibody Summary

Immunogen
This antibody has been engineered to specifically recognize the recombinant protein POLG2 using the following amino acid sequence: MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLG2
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 µg/ml
  • Western Blot 0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for POLG2 Antibody

  • DNA polymerase gamma accessory 55 kDa subunit
  • DNA polymerase subunit gamma-2, mitochondrial
  • EC 2.7.7.7
  • HP55
  • Mitochondrial DNA polymerase accessory subunit
  • mitochondrial DNA polymerase subunit gamma-2
  • mitochondrial DNA polymerase, accessory subunit
  • MtPolB
  • MTPOLBPOLG-BETA
  • p55
  • PEOA4
  • POLB
  • POLGB
  • PolG-beta
  • polymerase (DNA directed), gamma 2, accessory subunit

Background

The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit (POLG1; MIM 174763) and a 55-kD accessory subunit (POLG2).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol POLG2