Home » PLZF » PLZF Lysates » PLZF Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

PLZF Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: PLZF Overexpression Lysate (Adult Normal) [NBP2-10434] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLZF.
Western Blot: PLZF Overexpression Lysate (Adult Normal) [NBP2-10434] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLZF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

PLZF Overexpression Lysate Summary

Description

PLZF Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206745

Accession#: NM_006006

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
ZBTB16

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
74.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for PLZF Overexpression Lysate

  • PLZF
  • PLZFzinc finger and BTB domain-containing protein 16
  • ZBTB16
  • zinc finger and BTB domain containing 16
  • zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia)
  • Zinc finger protein 145
  • Zinc finger protein PLZF
  • ZNF145
  • ZNF145Promyelocytic leukemia zinc finger protein

Background

ZBTB16 - zinc finger and BTB domain containing 16

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

RAR alpha/NR1B1
Antibody (763)
NB200-322
Western Blot RAR alpha/NR1B1 Antibody (763)
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, WB
PML Protein
Antibody - BSA Free
NB100-59787
Western Blot PML Protein Antibody - BSA Free
Immunohistochemistry PML Protein Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence PML Protein Antibody - BSA Free
(1 Review)
(42 Publications)
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, PLA, WB
Bcl-6 Antibody
NBP2-59786
Immunocytochemistry/ Immunofluorescence Bcl-6 Antibody
Immunohistochemistry-Paraffin Bcl-6 Antibody
Immunohistochemistry-Paraffin Bcl-6 Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NCOR2 Antibody
NB120-5802
Immunocytochemistry/ Immunofluorescence NCOR2 Antibody
Immunocytochemistry/ Immunofluorescence NCOR2 Antibody
Immunocytochemistry/ Immunofluorescence NCOR2 Antibody
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
NCOR1 Antibody
(7A7A9) - BSA Free
NBP1-28863
Immunohistochemistry-Paraffin NCOR1 Antibody (7A7A9) - BSA Free
Immunohistochemistry NCOR1 Antibody (7A7A9) - BSA Free
Western Blot NCOR1 Antibody (7A7A9) - BSA Free
(3 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NPM1 Antibody -
BSA Free
NB110-61646
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Western Blot NPM1 Antibody - BSA Free
(2 Reviews)
(4 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
TZFP Antibody
NBP1-79984
Western Blot TZFP Antibody
Western Blot TZFP Antibody
Western Blot TZFP Antibody
Species: Hu
Applications: WB
HDAC1 Antibody
NB100-56340
Knockout Validated HDAC1 Antibody
Simple Western HDAC1 Antibody
Western Blot HDAC1 Antibody
(2 Reviews)
(19 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NuMA Antibody
NB500-174
Immunocytochemistry/ Immunofluorescence NuMA Antibody
Immunocytochemistry/ Immunofluorescence NuMA Antibody
Western Blot NuMA Antibody
(27 Publications)
Species: Hu, Ma, Mar, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Oct-3/4 Antibody
[Unconjugated]
AF1759
Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]
Immunocytochemistry Oct-3/4 Antibody [Unconjugated]
Simple Western Oct-3/4 Antibody [Unconjugated]
(77 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
GDNF [Unconjugated]
212-GD
Bioactivity GDNF [Unconjugated]
SDS-PAGE GDNF [Unconjugated]
SEC-MALS GDNF [Unconjugated]
(218 Publications)
Species: Hu
Applications: Bind, BA
c-Myc Antibody
(9E10) - BSA Free
NB600-302
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Western Blot c-Myc Antibody (9E10) - BSA Free
(4 Reviews)
(46 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
Cyclin A2 Antibody
NBP1-31330
Western Blot Cyclin A2 Antibody
Western Blot Cyclin A2 Antibody
Immunocytochemistry/ Immunofluorescence Cyclin A2 Antibody
(3 Reviews)
(6 Publications)
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
PLZF
Overexpression Lysate
NBP2-10434
PLZF Overexpression Lysate
Species: Hu
Applications: WB

Publications for PLZF Lysate (NBP2-10434) (0)

There are no publications for PLZF Lysate (NBP2-10434).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PLZF Lysate (NBP2-10434) (0)

There are no reviews for PLZF Lysate (NBP2-10434). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PLZF Lysate (NBP2-10434) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PLZF Products

Research Areas for PLZF Lysate (NBP2-10434)

Find related products by research area.

Blogs on PLZF

There are no specific blogs for PLZF, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PLZF Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol ZBTB16