![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p < 0.05, ** p < 0.01, and *** p < 0.001. (c) The blots show the protein expression levels in MRC-5 and HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26339636) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0021.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured by RNA-seq, qRT-PCR, and Western Blots. (a) CCNA2 is commonly downregulated. The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: * p < 0.05, ** p < 0.01, and *** p < 0.001. (c) The blots show the protein expression levels in MRC-5 and HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26339636) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary. |
| Localization | Nucleus, Cytoplasm |
| Predicted Species | Rhesus Macaque (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCNA2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Cyclin A2 antibody validated for IHC-P, WB from verified customer reviews. |
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| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 10/04/2015 |
Summary
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reviewed by:
Verified Customer |
IHC-P | Human | 10/02/2015 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 09/19/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin A2 Antibody (NBP1-31330)Find related products by research area.
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| Verified Customer 10/04/2015 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 10/02/2015 |
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| Application: | IHC-P | |
| Species: | Human |
| Verified Customer 09/19/2014 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | CCNA2 |
![Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody [NBP1-31330] - Detects Cyclin A2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31330-img0020.jpg "Immunocytochemistry/Immunofluorescence: Cyclin A2 Antibody [NBP1-31330] - Detects Cyclin A2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - 293T cells stained with Cyclin A2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunofluorescence-NBP1-31330-img0010.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - 293T cells stained with Cyclin A2 antibody. Image from verified customer review.")
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0006.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.")
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0007.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.")
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0013.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.")
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0014.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0016.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0019.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NBP1-31330-img0004.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of Cyclin A2 protein by 2. 5 ug of Cyclin A2 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunoprecipitation-NBP1-31330-img0009.jpg "Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of Cyclin A2 protein by 2. 5 ug of Cyclin A2 antibody 10% SDS-PAGE gel.")
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured by RNA-seq, qRT-PCR, & Western Blots. (a) Four genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log 2− delta delta CT; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: ∗p < 0.05, ∗∗p < 0.01, & ∗∗∗p < 0.001. (c) The blots show the protein expression levels in MRC-5 & HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26339636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-3102024161600.jpg "Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured by RNA-seq, qRT-PCR, & Western Blots. (a) Four genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log 2− delta delta CT; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: ∗p < 0.05, ∗∗p < 0.01, & ∗∗∗p < 0.001. (c) The blots show the protein expression levels in MRC-5 & HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26339636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Western_Blot_5394.jpg)
![Immunohistochemistry Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Immunohistochemistry_6819.jpg)
![Simple Western Cyr61/CCN1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyr61_AF4055_Simple_Western_20498.jpg)
![Immunohistochemistry Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Immunohistochemistry_11792.jpg)
![Western Blot Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Western_Blot_9526.jpg)
![Simple Western Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Simple_Western_20094.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9968.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9969.jpg)
![Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg)
![Immunohistochemistry NOV/CCN3 Antibody [Unconjugated]](https://images.novusbio.com/images2/NOV_AF1640_Immunohistochemistry_7314.jpg)
![Western Blot Cyclin A1 Antibody (722407) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_A1_MAB7046_Western_Blot_11398.jpg)
![Immunohistochemistry Cyclin A1 Antibody (722407) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_A1_MAB7046_Immunohistochemistry_11411.jpg)
![Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg)
![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP1-31330_10226.jpg)