Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) followed by HRP-conjugated Anti-Sheep ...read more
PLUNC was detected in immersion fixed frozen sections of mouse embryo nose (13 d.p.c.) using Sheep Anti-Mouse PLUNC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4274) at 1.7 µg/mL overnight at 4 °C. ...read more
A1AT treatment enhances SPLUNC1 and reduces NE activity in bronchoalveolar lavage (BAL) fluid of PA-infected WT mice. PA-infected mice were treated with A1AT for 22 hrs and sacrificed after 24 hrs of infection as ...read more
Pseudomonas aeruginosa (PA) infection reduces SPLUNC1 and increases neutrophil elastase (NE) activity in bronchoalveolar lavage (BAL) fluid of wild-type (WT) mice. BAL fluid from WT mice was processed for SPLUNC1 ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for PLUNC Antibody
bA49G10.5
BPIFA1
ligand-binding protein RYA3
LPLUNC3
Lung-specific protein X
LUNX
LUNXNASG
Nasopharyngeal carcinoma-related protein
Palate lung and nasal epithelium clone protein
palate, lung and nasal epithelium associated
palate, lung and nasal epithelium carcinoma associated
PLUNC
protein Plunc
Secretory protein in upper respiratory tracts
SPLUNC1
SPLUNC1SPURT
tracheal epithelium enriched protein
Tracheal epithelium-enriched protein
Von Ebner protein Hl
Background
PLUNC (Palate, lung, and nasal epithelium clone; also SPLUNC1) is a 25-30 kDa member of the PLUNC family, BPI/PLUNC/LBP superfamily of proteins. PLUNC is a secreted glycoprotein that is expressed by both adult and embryonic respiratory epithelium in the nose, trachea and bronchi/primary bronchioles. It serves as a natural antibacterial polypeptide in the respiratory system. Mature mouse PLUNC is 259 amino acids (aa) in length. It contains four, six-amino acid repeats (GxxLPL) over aa 23-52. There is reportedly one splice variant that shows a 28 aa substitution for the C-terminal 45 amino acids (aa 234-278). Over aa 59-278, mouse PLUNC shares 91% and 75% aa identity with rat and human PLUNC, respectively.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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