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EHHADH Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915] - Staining in human liver and cerebral cortex tissues using anti-EHHADH antibody. Corresponding EHHADH RNA-seq data are presented ...read more
Western Blot: EHHADH Antibody [NBP1-84915] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915] - Staining of human liver shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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EHHADH Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TSGRRILADEALKLGILDKVVNSDPVEEAIRFAQRVSDQPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQAAVQYPYEVG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EHHADH
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
EHHADH Protein (NBP1-84915PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EHHADH Antibody

  • enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
  • enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
  • LBFP
  • LBP
  • L-PBE
  • PBFEMGC120586
  • peroxisomal bifunctional enzyme3,2-trans-enoyl-CoA isomerase
  • peroxisomal enoyl-CoA hydratase
  • peroxisomal

Background

The protein encoded by the EHHADH gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomalbeta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while theC-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomaldisorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for thisgene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EHHADH