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PKC delta [p Ser645] Antibody - BSA Free

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Immunohistochemistry-Paraffin: PKC delta [p Ser645] Antibody [NB100-82141] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82141 (left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry-Paraffin: PKC delta [p Ser645] Antibody [NB100-82141] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82141 (left) or the same antibody preincubated ...read more
Western Blot: PKC delta [p Ser645] Antibody [NB100-82141] - Analysis of extracts from PC12 cells untreated or treated with PMA using PKCd(Phospho-Ser645) antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB100-82141-0.025ml
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PKC delta [p Ser645] Antibody - BSA Free Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PKC delta around the phosphorylation site of serine 645 (R-L-SP-Y-S).
Modification
p Ser645
Specificity
Detects endogenous levels of PKC delta only when phosphorylated at serine 645.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCD
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PKC delta Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKC delta [p Ser645] Antibody - BSA Free

  • EC 2.7.11
  • EC 2.7.11.13
  • MAY1
  • MGC49908
  • nPKC-delta
  • PKC delta
  • PKCD
  • PKCy
  • pPKCY
  • PRKCD
  • protein kinase C delta type
  • protein kinase C delta VIII
  • protein kinase C, delta

Background

Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

PKC delta Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKCD
Entrez
Uniprot