I'm planning to use PKC alpha Antibody (MC5) (NB600-201) for my next research. I'd like to know how many antibody kit I'll need for my sample (approx. 90 specimens).

Name: PKC alpha Antibody (MC5)
Brand: Novus Biologicals
SKU: NB600-201
Rating: 5 (1 reviews)

Question

Accepted Answer

Unfortunately, we have neither an ELISA kit for PKC alpha nor a PKC alpha extraction kit. The PKC alpha antibodies we supply are simply the primary antibodies, with no other reagents. However, we do also sell secondary antibodies, some of which are already conjugated, and antibody labeling kits. I'm afraid all I can suggest is that you consult the literature for more information on your application.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Bv, Ca, Ch, Fi, Pm, Pm, Rb, Sh, ZeSpecies Glossary
Applications	WB, ELISA, Flow, Func, ICC/IF, IHC, IP
Clone	MC5
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB600-201AF350

Alexa Fluor 405 NB600-201AF405

Alexa Fluor 488 NB600-201AF488

Alexa Fluor 532 NB600-201AF532

Alexa Fluor 594 NB600-201AF594

Alexa Fluor 647 NB600-201AF647

Alexa Fluor 700 NB600-201AF700

Alexa Fluor 750 NB600-201AF750

Allophycocyanin NB600-201APC

Allophycocyanin/Cy7 NB600-201APCCY7

Biotin NB600-201B

DyLight 350 NB600-201UV

DyLight 405 NB600-201V

DyLight 488 NB600-201G

DyLight 550 NB600-201R

DyLight 594 NB600-201DL594

DyLight 650 NB600-201C

DyLight 680 NB600-201FR

DyLight 755 NB600-201IR

FITC NB600-201F

HRP NB600-201H

Janelia Fluor 549 NB600-201JF549

Janelia Fluor 646 NB600-201JF646

PE NB600-201PE

PE/Atto594 NB600-201PEATT594

PE/Cy5.5 NB600-201PECY55

PE/Cy7 NB600-201PECY7

PerCP NB600-201PCP

mFluor Violet 450 SE NB600-201MFV450

mFluor Violet 500 SE NB600-201MFV500

mFluor Violet 610 SE NB600-201MFV610

Catalog# & Formulation	Size	Price
Azide and BSA Free NBP2-80911

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-201AF350
Alexa Fluor 405	NB600-201AF405
Alexa Fluor 488	NB600-201AF488
Alexa Fluor 532	NB600-201AF532
Alexa Fluor 594	NB600-201AF594
Alexa Fluor 647	NB600-201AF647
Alexa Fluor 700	NB600-201AF700
Alexa Fluor 750	NB600-201AF750
Allophycocyanin	NB600-201APC
Allophycocyanin/Cy7	NB600-201APCCY7
Biotin	NB600-201B
DyLight 350	NB600-201UV
DyLight 405	NB600-201V
DyLight 488	NB600-201G
DyLight 550	NB600-201R
DyLight 594	NB600-201DL594
DyLight 650	NB600-201C
DyLight 680	NB600-201FR
DyLight 755	NB600-201IR
FITC	NB600-201F
HRP	NB600-201H
Janelia Fluor 549	NB600-201JF549
Janelia Fluor 646	NB600-201JF646
PE	NB600-201PE
PE/Atto594	NB600-201PEATT594
PE/Cy5.5	NB600-201PECY55
PE/Cy7	NB600-201PECY7
PerCP	NB600-201PCP
mFluor Violet 450 SE	NB600-201MFV450
mFluor Violet 500 SE	NB600-201MFV500
mFluor Violet 610 SE	NB600-201MFV610

PKC alpha Antibody (MC5)

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Publications for PKC alpha Antibody (NB600-201)(27)

Review for PKC alpha Antibody (NB600-201) (1) 51

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Immunogen	Purified bovine brain Protein Kinase C
Specificity	This antibody is capable of detecting PKC beta as well.
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	PRKCA
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Tris-Glycine and 0.15 M NaCl
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein A purified

Publications using NB600-201	Applications	Species
Bernardo-Col�n A, Dong L, Abu-Asab M et al. Ablation of Pigment Epithelium-derived Factor Receptor (PEDF-R/Pnpla2) Causes Photoreceptor Degeneration Journal of lipid research 2023-03-17 [PMID: 36934843] (IHC-P, Mouse) Details: Dilution: 1:50	IHC-P	Mouse
Ren J, Hu Z, Li Q et al. Temperature-induced embryonic diapause in chickens is mediated by PKC-NF-?B-IRF1 signaling BMC biology 2023-03-08 [PMID: 36882743] (ICC/IF, Chicken)	ICC/IF	Chicken
Antoniali G, Dalla E, Mangiapane G et al. APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b Cellular and molecular life sciences : CMLS 2022-07-25 [PMID: 35876890] (WB)	WB
Yamasaki S, Tu H, Matsuyama T et al. A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation iScience 2022-01-01 [PMID: 35024589] (IHC-Fr, Monkey)	IHC-Fr	Monkey
Xie Z, Wang Q, Hu S. Coordination of PRKCA/PRKCA-AS1 interplay facilitates DNA methyltransferase 1 recruitment on DNA methylation to affect protein kinase C alpha transcription in mitral valve of rheumatic heart disease Bioengineered 2021-09-06 [PMID: 34482802]
Matsuyama T, Tu HY, Sun J Et al. Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation iScience 2021-08-20 [PMID: 34409267]
Della Santina L, Yu A, Harris S, et al. Disassembly and Rewiring of a Mature Converging Excitatory Circuit Following Injury Cell Rep 2021-08-04 [PMID: 34348156]
Chen Aw, Biggar K, Nygard K Et Al. IGFBP-1 hyperphosphorylation in response to nutrient deprivation is mediated by activation of protein kinase Calpha (PKCalpha) Molecular and cellular endocrinology 2021-07-24 [PMID: 34314739] (IHC-P, WB)	IHC-P, WB
Thomson B, Grannonico M, Liu F et Al. Angiopoietin-1 Knockout Mice as a Genetic Model of Open-Angle Glaucoma Transl Vis Sci Technol 2020-08-21 [PMID: 32818103]
Grillo SL, McDevitt DS, Voas MG et al. Adenosine receptor expression in the adult zebrafish retina Purinergic Signal 2019-07-06 [PMID: 31273575] (IF/IHC, Zebrafish)	IF/IHC	Zebrafish
Lin YJ, Liang WM, Chen CJ et al. Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells Phytomedicine 2019-03-13 [PMID: 30901663] (WB, Human)	WB	Human
Neuhauss S, Haug M, Gesemann M, Berger M. Differential expression of PKCa and -B in the zebrafish retina. bioRxiv 2018-07-03 [PMID: 30604284] (IF/IHC, Zebrafish)	IF/IHC	Zebrafish
Hellmer CB, Clemons MR, Nawy S, Ichinose T. A group I metabotropic glutamate receptor controls synaptic gain between rods and rod bipolar cells in the mouse retina. Physiol Rep. 2018-10-01 [PMID: 30338673] (IF/IHC, Mouse)	IF/IHC	Mouse
Xie J, Huo S, Li Y et al. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Muller Cell Notch Signaling Pathway Cell Transplant. 2017-06-09 [PMID: 28185609] (ICC/IF, Rat)	ICC/IF	Rat
Guimaraes-Souza EM, Perche O, Morgans CW et al. Fragile X Mental Retardation Protein expression in the retina is regulated by light. Exp Eye Res 2016-05-01 [PMID: 26719241]
Zimov S, Yazulla S. Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells. Cell Tissue Res 2008-07-01 [PMID: 18449566]
Quraishi S, Gayet J, Morgans CW et al. Distribution of group-III metabotropic glutamate receptors in the retina. J Comp Neurol;501(6):931-43. 2007-04-20 [PMID: 17311335]
Bayley PR, Morgans CW. Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina. J Comp Neurol;500(2):286-98. 2007-01-10 [PMID: 17111373]
Ng T, Parsons M, Hughes WE, et al. Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility. EMBO J. 2001-06-01 [PMID: 11387207] (WB, IP, ICC/IF, Human)	WB, IP, ICC/IF	Human
Cesare P, Dekker LV, Sardini A et al. pecific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat. Neuron. 1999-07-01 [PMID: 10433272] (WB, Rat)	WB	Rat
Ricciarelli R, Tasinato A, Clement S et al. alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state. Biochem J. 1998-08-15 [PMID: 9693126] (WB, Rat)	WB	Rat
Gatti A, Robinson PJ. . Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon. Eur J Biochem. 1997-10-01 [PMID: 9363758] (WB, Rat)	WB	Rat
Clement S, Tasinato A, Boscoboinik d et al. The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation. Eur J Biochem. 1997-06-15 [PMID: 9219534] (WB, Rat)	WB	Rat
Gatti A, Robinson PJ. . Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation. J Biol Chem. 1996-12-06 [PMID: 8940195] (WB, Rat)	WB	Rat
Wyss-Coray T, Masliah E, Toggas SM et al. Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis. J Clin Invest. 1996-02-01 [PMID: 8609236] (ICC/IF, Mouse, Human)	ICC/IF	Mouse, Human
Escriba PV, Sastre M, Garcia-Sevilla JA. Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures. Proc Natl Acad Sci USA. 1995-08-01 [PMID: 7638236] (WB, Rat)	WB	Rat
Lacal JC, Cuadrado A, Jones JE et al. Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells. Mol Cell Biol. 1990-06-01 [PMID: 2188105] (WB, Human)	WB	Human
Show All 27 Publications. Collapse Publications.

Gene Symbol	PRKCA
Entrez	282001(Bovine) 5578(Human) 75292(Mouse) 313834(Rat)
Uniprot	P17252() P04409(Bovine)

	Verified Customer 07/30/2021
Application:	IHC-P
Species:	Mouse