cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely related. PKA mediates a variety of diverse cellular responses (cell growth and proliferation, ion transport, triglyceride storage and metabolism regulation, embryonic development, and gene transcription) in virtually all eukaryotic systems via phosphorylation of a wide range of downstream target proteins in both they cytoplasm and nucleus. Elevation of cAMP triggers the following cascade of events: PKA translocation from the cytoplasm to the nucleus, transcription factor cAMP response element binding protein (CREB) phosphorylation, TFIIB binding to the TATA-box-binding protein TBP1, and lastly, phosphorylated CREB binding to the pol II transcription initiation complex.
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Immunocytochemistry/Immunofluorescence: PKC alpha Antibody
A review by Roberts highlights the role of cAMP signaling in the vasculature and inflammation using a PKA C-alpha antibody as an investigative tool1. Additionally, recent interesting work is being done by Castro’s group, where they are exploring the role of cAMP and PKA in neurons using FRET optical biosensors that collect both spatial and temporal profiles using a PKA C-alpha antibody2. These researchers are hoping that this technique will allow for more detailed and better understanding of neuropsychosis drug MOA and the development of novel molecules. Work from Enns’ lab at the University of Washington used immunoblotting with the PKA C-alpha antibody to follow age-related metabolic defects in mice3. The metabolic phenotypes generated from their data help shed pharmacological insights on aging with regards to obesity, altered body fat distribution, and insulin resistance. Novus Biologicals offers PKC Alpha reagents for your research needs including:
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