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PKC alpha Antibody (MC5) [PE]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fi, Pm, Pm, Rb, Sh, ZeSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC, IP
Clone
MC5
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-201AF350
Alexa Fluor 405
NB600-201AF405
Alexa Fluor 488
NB600-201AF488
Alexa Fluor 532
NB600-201AF532
Alexa Fluor 594
NB600-201AF594
Alexa Fluor 647
NB600-201AF647
Alexa Fluor 700
NB600-201AF700
Alexa Fluor 750
NB600-201AF750
Allophycocyanin
NB600-201APC
Allophycocyanin/Cy7
NB600-201APCCY7
Biotin
NB600-201B
CoraFluor 1
NB600-201CL1
DyLight 350
NB600-201UV
DyLight 405
NB600-201V
DyLight 488
NB600-201G
DyLight 550
NB600-201R
DyLight 594
NB600-201DL594
DyLight 650
NB600-201C
DyLight 680
NB600-201FR
DyLight 755
NB600-201IR
FITC
NB600-201F
HRP
NB600-201H
Janelia Fluor 525
NB600-201JF525
Janelia Fluor 549
NB600-201JF549
Janelia Fluor 646
NB600-201JF646
Janelia Fluor 669
NB600-201JF669
PE/Atto594
NB600-201PEATT594
PE/Cy5.5
NB600-201PECY55
PE/Cy7
NB600-201PECY7
PerCP
NB600-201PCP
Unconjugated
NB600-201
mFluor Violet 450 SE
NB600-201MFV450
mFluor Violet 500 SE
NB600-201MFV500
mFluor Violet 610 SE
NB600-201MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB600-201
Azide and BSA Free
NBP2-80911
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PKC alpha Antibody (MC5) [PE] Summary

Immunogen
Purified bovine brain Protein Kinase C
Specificity
This antibody is capable of detecting PKC beta as well.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRKCA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Radioimmunoassay
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:35024589) Use in Baboon reported in scientific literature (PMID:34314739) Zebrafish reactivity reported in scientific literature (PMID: 31273575)..

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for PKC alpha Antibody (MC5) [PE]

  • AAG6
  • aging-associated gene 6
  • EC 2.7.11
  • EC 2.7.11.13
  • MGC129901
  • PKC alpha
  • PKCA
  • PKC-A
  • PKC-alpha
  • PKCAMGC129900
  • PRKACA
  • PRKCA
  • protein kinase C alpha type
  • protein kinase C, alpha

Background

Protein Kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanisms. PKC alpha belongs to the conventional PKC group, alongside Beta-I, Beta-II, and Gamma, and is activated by phosphatidylserine in a calcium dependent manner. Furthermore, PKC alpha is localized to the membrane and cytoplasm, but in some cases can translocate to the nucleus. PKC alpha helps to positively and negatively regulate cell proliferation, apoptosis, differentiation, adhesion, and migration via phosphorylation of RAF1, BCL2, CSPG4, TNNT2/CTNT, or MAPK1/3. It also exhibits positive interactions with ADAP1/CENTA1, CSPG4 and PRKCABP. PKC alpha demonstrates anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3 (PMCID: PMC1360842). Additional research has shown PKC alpha's involvement in cellular inflammation (PMCID: PMC3080812).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKC alpha Antibody (NB600-201PE). (Showing 1 - 1 of 1 FAQ).

  1. We are looking into protein kinase C-alpha antibodies for immunohistochemistry on formalin fixed, paraffin embedded tissue. I see that you have many antibodies, including several monoclonals. I wanted to confirm with you that the following antibodies are specific for C-alpha. NB600-201=MC5 (an older paper suggested that this might react with alpha, beta, gamm), NB110-57356=Y124, NB110-57353=Y143. Also, if you have an comparator data or feedback regarding PKC-alpha antibodies for IHC on FFPE, that would be very helpful. We would like to pick 1 antibody that is easy to titer and robust, so that we can move forward with our studies.
    • We have never heard any feedback that these cross-react with other PKC proteins. If you have seen data that MC5 may cross-react, then I would consider avoiding this antibody if you are concerned. The immunogens for NB110-57356 and NB110-57353 come from the N- and C-terminals, which is where the PKC proteins vary the most, so these are not expected to cross-react and we have had no feedback or testing data that suggests that they do. I would recommend NB110-57356, since it has been published with. You may want to review the published data to determine if it seems this antibody will suit your needs.

Secondary Antibodies

 

Isotype Controls

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There is nothing beta than PKC Alpha
cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel...  Read full blog post.
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Bioinformatics

Gene Symbol PRKCA