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PDGFR alpha Antibody (8E12F2) - BSA Free

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Western Blot: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis using PDGFRA mAb against HEK293 (1) and PDGFRA (AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis using PDGFRA mAb against HEK293 (1) and PDGFRA (AA: 361-528)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) [NBP2-52528] - PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Imaging of Optice Nerve. Primary Antibody (red, dilution1:800) and DAPI (blue). This ...read more
Flow (Intracellular): PDGF R alpha Antibody (8E12F2) [NBP2-52528] - An intracellular stain was performed on HeLa cells with PDGF R alpha Antibody (8E12F2) NBP2-52528AF488 (blue) and a matched isotype control (orange). ...read more
Western Blot: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis using PDGFRA mAb against human PDGFRA (AA: 361-528) recombinant protein. (Expected MW is 44.8 kDa)
Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (8E12F2) [NBP2-52528] - PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis of Hela cells using PDGFRA 8E12F2 HELA 100 mouse mAb (green). Blue: DRAQ5 ...read more
Flow Cytometry: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Analysis of Hela cells using PDGFRA mouse mAb (green) and negative control (red).
ELISA: PDGF R alpha Antibody (8E12F2) [NBP2-52528] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity Hu, FeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, CyTOF-ready
Clone
8E12F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52528-0.025mg
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PDGFR alpha Antibody (8E12F2) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PDGFR alpha (AA: 361-528) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDGFRA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
122.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3
using
NBP2-52528 in the following applications:

Reactivity Notes

Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PDGFR alpha Antibody (8E12F2) - BSA Free

  • alpha-type platelet-derived growth factor receptor
  • CD140 antigen-like family member A
  • CD140a antigen
  • CD140a
  • EC 2.7.10
  • EC 2.7.10.1
  • MGC74795
  • PDGF R alpha
  • PDGFR alpha
  • PDGFR2
  • PDGFRA
  • PDGFRA/BCR fusion
  • PDGF-R-alpha
  • platelet-derived growth factor receptor, alpha polypeptide
  • rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
  • RHEPDGFRA

Background

Platelet-derived growth factor receptors exhibit tyrosine-protein kinase activity and have been implicated in the control of cell proliferation, survival and migration. PDGF receptors, PDGFR-alpha and PDGFR-beta, have 5 extracellular immunoglobulin-like domains and an intracellular tyrosine kinase domain. Upon binding a PDGF, the receptors form homo-and heterodimers. Dimerization of the receptors results in phosphorylation in the complex. More than 10 different SH2-domain-containing molecules have been shown to bind to different autophosphorylation sites in the PDGF-alpha and beta receptors. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDGFR alpha Antibody (NBP2-52528) (0)

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Reviews for PDGFR alpha Antibody (NBP2-52528) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Feline.

Reviews using NBP2-52528:
Filter by Applications
ICC
(2)
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Filter by Species
Feline
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry PDGFR alpha NBP2-52528
Enlarge 		3
reviewed by:
Verified Customer
 ICC Feline 08/08/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedOptic nerve
SpeciesFeline
Lot140425
  3
reviewed by:
Kazuya Oikawa
 ICC Feline 05/28/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedOptic nerve
SpeciesFeline
Lot140425

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDGFR alpha Antibody (NBP2-52528) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Immunocytochemistry PDGFR alpha NBP2-52528
Enlarge
 Verified Customer
08/08/2017
Application: ICC
Species: Feline
read this review
Immunocytochemistry PDGFR alpha NBP2-52528
Enlarge
 Kazuya Oikawa
05/28/2017
Application: ICC
Species: Feline
read this review

Bioinformatics

Gene Symbol PDGFRA