![Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0023.jpg "Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Pax6. The exact sequence is proprietary. |
| Localization | Nucleus |
| Marker | Neural Marker |
| Predicted Species | Porcine (100%), Bovine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (94%), Chicken (98%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PAX6 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Western_Blot_5161.jpg)
![Chromatin Immunoprecipitation (ChIP) SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Chromatin_Immunoprecipitation_11224.jpg)
![Simple Western SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Simple_Western_16676.jpg)
| Publications using NBP2-19711 | Applications | Species |
|---|---|---|
| Larrigan S, Joshi S, Mattar P Divergent phenotypes in germline versus conditional mutant mouse models of Sifrim-Hitz-Weiss Syndrome bioRxiv 2023-02-16 (IHC-Fr, Mouse) | IHC-Fr | Mouse |
| Larrigan S, Joshi SV, Mattar P Divergent phenotypes in constitutive versus conditional mutant mouse models of Sifrim-Hitz-Weiss syndrome Human molecular genetics 2023-09-21 [PMID: 37738575] | ||
| Wen Y, Jin S. Production of neural stem cells from human pluripotent stem cells J. Biotechnol. 2014-08-20 [PMID: 25150215] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Pax6 Antibody (NBP2-19711)Find related products by research area.
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Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP2-19711] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAX6 protein stained by PAX6 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19711-img0013.jpg "Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP2-19711] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAX6 protein stained by PAX6 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Mouse retina. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP2-19711-img0021.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Mouse retina. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Pax6 Antibody [NBP2-19711] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0015.jpg "Western Blot: Pax6 Antibody [NBP2-19711] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Pax6 Antibody [NBP2-19711] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0017.jpg "Western Blot: Pax6 Antibody [NBP2-19711] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0010.jpg "Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0012.jpg "Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody diluted at 1:250.")
![Immunoprecipitation: PAX6 Antibody [NBP2-19711] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ug of PAX6 antibody 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PAX6-Antibody-Immunoprecipitation-NBP2-19711-img0004.jpg "Immunoprecipitation: PAX6 Antibody [NBP2-19711] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ug of PAX6 antibody 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420104458.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202419593540.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420115034.jpg "Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242075957.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242001050.jpg "Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Pax6 Antibody [NBP2-19711] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-161020241941454.jpg "Western Blot: Pax6 Antibody [NBP2-19711] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunocytochemistry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images2/Nestin_MAB1259_Immunocytochemistry_6561.jpg)
![Intracellular Staining by Flow Cytometry Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/antibody/mab1259_human-nestin-mab-clone-196908-intracellular-staining-by-flow-cytometry-24120239959.jpg)
![Western Blot Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/mab1259_human-nestin-mab-clone-196908-41202410481129.jpg)
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464SH_2840.jpg)
![SDS-PAGE Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464-SH_341.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot PDX-1/IPF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PDX-1_AF2419_Western_Blot_5187.jpg)
![Immunocytochemistry PDX-1/IPF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PDX1_AF2419_Immunocytochemistry_16575.jpg)
![Simple Western PDX-1/IPF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PDX1_AF2419_Simple_Western_16996.jpg)
![Simple Western Islet-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Islet1_AF1837_Simple_Western_21249.jpg)
![Western Blot Islet-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Islet1_AF1837_Western_Blot_21254.jpg)
![Immunocytochemistry Islet-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Islet1_AF1837_Immunocytochemistry__Immunofluorescence_17786.jpg)
![Immunocytochemistry NeuroD1 Antibody [Unconjugated]](https://images.novusbio.com/images2/NeuroD1_AF2746_Immunocytochemistry_7291.jpg)
![Immunocytochemistry Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax2_AF3364_Immunocytochemistry_14405.jpg)
![Western Blot Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/af3364_human-pax2-affinity-purified-polyclonal-ab-western-blot-145202183852.jpg)
![Immunohistochemistry Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/af3364_human-pax2-affinity-purified-polyclonal-ab-immunohistochemistry-192021142455.jpg)
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Immunocytochemistry Glucagon Antibody (181402) [Unconjugated]](https://images.novusbio.com/images2/Glucagon_MAB1249_Immunocytochemistry_6560.jpg)
![Intracellular Staining by Flow Cytometry Glucagon Antibody (181402) [Unconjugated]](https://images.novusbio.com/images2/Glucagon_MAB1249_Flow_Cytometry_20264.jpg)
![Western Blot Neurogenin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neurogenin-3_AF3444_Western_Blot_5300.jpg)
![Immunohistochemistry Neurogenin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/af3444_human-neurogenin-3-affinity-purified-polyclonal-ab-immunohistochemistry-192021141921.jpg)
![Immunocytochemistry Otx2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Otx2_AF1979_Immunocytochemistry_11033.jpg)
![Immunohistochemistry Otx2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Otx2_AF1979_Immunohistochemistry_22974.jpg)
![Simple Western Otx2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Otx2_AF1979_Simple_Western_20223.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")