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Pax6 Antibody (PAX6/7078R)

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Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/7078R) [NBP3-13988] - Formalin-fixed, paraffin-embedded human pancreas stained with Pax6 antibody (PAX6/7078R). Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: Pax6 Antibody (PAX6/7078R) [NBP3-13988] - Formalin-fixed, paraffin-embedded human pancreas stained with Pax6 antibody (PAX6/7078R). Inset: PBS instead of primary antibody; secondary only ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
PAX6/7078R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14033AF350
Alexa Fluor 405
NBP3-14033AF405
Alexa Fluor 488
NBP3-14033AF488
Alexa Fluor 532
NBP3-14033AF532
Alexa Fluor 594
NBP3-14033AF594
Alexa Fluor 647
NBP3-14033AF647
Alexa Fluor 700
NBP3-14033AF700
Alexa Fluor 750
NBP3-14033AF750
Allophycocyanin
NBP3-14033APC
Biotin
NBP3-14033B
CoraFluor 1
NBP3-14033CL1
DyLight 350
NBP3-14033UV
DyLight 405
NBP3-14033V
DyLight 488
NBP3-14033G
DyLight 550
NBP3-14033R
DyLight 594
NBP3-14033DL594
DyLight 650
NBP3-14033C
DyLight 680
NBP3-14033FR
DyLight 755
NBP3-14033IR
FITC
NBP3-14033F
HRP
NBP3-14033H
Janelia Fluor 525
NBP3-14033JF525
Janelia Fluor 549
NBP3-14033JF549
Janelia Fluor 585
NBP3-14033JF585
Janelia Fluor 635
NBP3-14033JF635
Janelia Fluor 646
NBP3-14033JF646
Janelia Fluor 669
NBP3-14033JF669
PE
NBP3-14033PE
PerCP
NBP3-14033PCP
mFluor Violet 450 SE
NBP3-14033MFV450
mFluor Violet 500 SE
NBP3-14033MFV500
mFluor Violet 610 SE
NBP3-14033MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-14033
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Pax6 Antibody (PAX6/7078R) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14033)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (N-terminus; aa 1-300) of human Pax6 protein (Uniprot: P26367)
Localization
Nuclear
Marker
Stem Cell Marker
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
PAX6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Pax6 Antibody (PAX6/7078R)

  • keratitis)
  • MGC17209
  • Oculorhombin
  • paired box 6
  • paired box protein Pax-6
  • Pax6

Background

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-13988
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pax6 Antibody (NBP3-13988). (Showing 1 - 1 of 1 FAQ).

  1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
    • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

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Bioinformatics

Gene Symbol PAX6
Uniprot