PACT Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

PACT (PKR Activator; also HSD14, PRKRA and RAX [in mouse]) is a 34-37 kDa member of the PRKRA family of molecules. It is a ubiquitously expressed, cytoplasmic, 313 amino acid (aa) protein that contains three discrete double-stranded (ds) RNA binding motifs (aa 31-101; 126-194; 240-308). It exists as a monomer, homodimer, and heterodimer with at least two partners, TRBP and Dicer. Two isoform variants are known to exist, including one that utilizes an alternative start site at Met26, and another that shows a 10 aa substitution for aa 1-21. PACT is perhaps best known for its relationship with PKR, a 52-58 kDa Ser/Thr kinase that downmodulates protein synthesis and initiates apoptosis. PKR is constitutively silent, and activated via two principal ways; the first is through binding to (viral) dsRNA, and the second is through binding to an activator, PACT. PACT is kept in check upstream through dimerization with at 48-49 kDa regulatory protein termed TRBP. Following growth factor withdrawl or exposure to bacterial products such as LPS, PACT is phosphorylated at Ser287, and this inactive PACT:TRBP heterodimer dissociates, freeing up PACT to bind to PKR. PACT's interaction with PKR induces an autophosphorylation event on PKR. This allows for an activated PKR interaction with eIF2a, causing its phosphorylation and a subsequent reduction of protein synthesis. PACT may also act independently of PKR, and serve as a component of RISC, a complex that downregulates mRNA translation. Within this complex, PACT likely associates with Dicer in concert with Argonaute. Full-length PACT shares 98% aa sequence identity with the mouse ortholog to human PACT termed RAX.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Array AF1980

• PACT Antibodies
• PACT Lysate
• PACT Proteins