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Ornithine Carbamoyltransferase Antibody (OTI8A1) - Azide and BSA Free

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Western Blot: Ornithine Carbamoyltransferase Antibody (OTI8A1) - Azide and BSA Free [NBP2-73149] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY OTC (RC214662, Right lane) cDNA ...read more
Immunohistochemistry: Ornithine Carbamoyltransferase Antibody (OTI8A1) - Azide and BSA Free [NBP2-73149] - Paraffin-embedded Carcinoma of Human liver tissue using anti-OTC mouse monoclonal antibody. Dilution: 1:150
Immunohistochemistry: Ornithine Carbamoyltransferase Antibody (OTI8A1) - Azide and BSA Free [NBP2-73149] - Paraffin-embedded Human liver tissue within the normal limits using anti OTC mouse monoclonal antibody. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI8A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Ornithine Carbamoyltransferase Antibody (OTI8A1) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 33-354 of human OTC (NP_000522)produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
OTC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Western Blot 1:2000
Theoretical MW
36.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Ornithine Carbamoyltransferase Antibody (OTI8A1) - Azide and BSA Free

  • EC 2.1.3
  • EC 2.1.3.3
  • MGC129967
  • MGC129968
  • MGC138856
  • OCTD
  • Ornithine Carbamoyltransferase
  • ornithine carbamoyltransferase, mitochondrial
  • Ornithine Transcarbamylase
  • OTC
  • OTCase

Background

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol OTC