![Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Western-Blot-NBP1-32732-img0008.jpg "Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length human NHP2L1 Recombinant protein. |
| Localization | Nucleus, nucleolus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SNU13 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in immunoprecipitation reported in scientific literature (PMID: 26106861). IHC-Wm Assay dependent. |
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| Theoretical MW | 14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP1-32732 | Applications | Species |
|---|---|---|
| Tresini M, Warmerdam DO, Kolovos P et al. The core spliceosome as target and effector of non-canonical ATM signalling. Nature 2015-07-02 [PMID: 26106861] (IP, Human) | IP | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for NHP2L1 Antibody (NBP1-32732)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32732-img0004.jpg "Immunocytochemistry/Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-32732-img0005.jpg "Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.")
![Western Blot: NHP2L1 Antibody [NBP1-32732] - Sample (30 ug of whole cell lysate)A: HeLaB: Hep G2 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Western-Blot-NBP1-32732-img0006.jpg "Western Blot: NHP2L1 Antibody [NBP1-32732] - Sample (30 ug of whole cell lysate)A: HeLaB: Hep G2 12% SDS PAGE, antibody diluted at 1:1000.")
![Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract.](http://images.novusbio.com/fullsize/NHP2L1-Antibody-Western-Blot-NBP1-32732-img0007.jpg "Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract.")
![NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed zebrafish embryos. NHP2L1 antibody [N1C3] dilution: 1:200.](http://images.novusbio.com/fullsize/antibody/nbp1-32732_rabbit-polyclonal-nhp2l1-antibody-immunohistochemistry-whole-mount-8520237549..jpg "NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed zebrafish embryos. NHP2L1 antibody [N1C3] dilution: 1:200.")
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![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")