![Western Blot: RUVBL1 Antibody [NBP2-20245] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:2500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP2-20245-img0014.jpg "Western Blot: RUVBL1 Antibody [NBP2-20245] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:2500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, Pm, RM, Xp, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary. |
| Localization | Nucleus matrix; Nucleus; nucleoplasm; Cytoplasm; Membrane |
| Predicted Species | Chimpanzee (100%), Bovine (99%), Rhesus Macaque (100%), Zebrafish (97%), Xenopus (97%), Chicken (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RUVBL1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
| Publication using NBP2-20245 | Applications | Species |
|---|---|---|
| Silva B, Pentz R, Figueira AM et al. Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein Viruses 2019-04-23 [PMID: 31018511] (WB, ICC/IF, KD, Human) | WB, ICC/IF, KD | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Agbolahan Sofela |
WB | Human | 09/30/2019 |
Summary
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Enlarge |
reviewed by:
Tetsuo Kiguchi |
IP | Human | 11/30/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for RUVBL1 Antibody (NBP2-20245)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Agbolahan Sofela 09/30/2019 |
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| Application: | WB | |
| Species: | Human |
| Tetsuo Kiguchi 11/30/2017 |
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| Application: | IP | |
| Species: | Human |
![Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20245-img0012.jpg "Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0010.jpg "Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Human colon carcinoma, using RUVBL1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0002.jpg "Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Human colon carcinoma, using RUVBL1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat heart. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0007.jpg "Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat heart. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse colon. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0008.jpg "Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse colon. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0009.jpg "Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunoprecipitation-NBP2-20245-img0006.jpg "Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - Endogenous RUVBL1 & RUVBL2 colocalize with HA-NP. HeLa cells were transfected with vector control, HA-NP, or HA-VP35. Twenty-four h later, the cells were fixed & processed for immunofluorescence detection of endogenous RUVBL1 or RUVBL2 in the presence of vector control, HA-NP, or HA-VP35. Representative images of (A) endogenous RUVBL1 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) & (B) endogenous RUVBL2 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) are shown. HA-NP or HA-VP35 (green), RUVBL1/2 (red), & Hoechst 33342 nuclear stain (blue) were visualized by confocal microscopy. Scale bars = 20 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20245_rabbit-polyclonal-ruvbl1-antibody-31020241534318.jpg "Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - Endogenous RUVBL1 & RUVBL2 colocalize with HA-NP. HeLa cells were transfected with vector control, HA-NP, or HA-VP35. Twenty-four h later, the cells were fixed & processed for immunofluorescence detection of endogenous RUVBL1 or RUVBL2 in the presence of vector control, HA-NP, or HA-VP35. Representative images of (A) endogenous RUVBL1 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) & (B) endogenous RUVBL2 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) are shown. HA-NP or HA-VP35 (green), RUVBL1/2 (red), & Hoechst 33342 nuclear stain (blue) were visualized by confocal microscopy. Scale bars = 20 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20245_rabbit-polyclonal-ruvbl1-antibody-91020242063352.jpg "Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:1000.")
![Western Blot Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Western_Blot_5182.jpg)
![Simple Western Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/16395.jpg)
![Immunocytochemistry Histone H2AX [p Ser139] Antibody [Unconjugated]](https://images.novusbio.com/images2/Histone_H2AX_AF2288_Immunocytochemistry__Immunofluorescence_16920.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
