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NOP58 Antibody

Images

 
Independent Antibodies: Western Blot: NOP58 Antibody [NBP1-81680] - Analysis using Anti-NOP58 antibody NBP1-81680 (A) shows similar pattern to independent antibody NBP1-81681 (B).
Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP1-81680] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680] - Staining of human stomach shows strong nucleolar and moderate nuclear positivity in glandular cells.
Western Blot: NOP58 Antibody [NBP1-81680] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680] - Staining of human cerebral cortex shows strong nucleolar and weaker nuclear positivity in neurons.
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680] - Staining of human kidney shows strong nucleolar positivity in cells in tubules.
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680] - Staining of human lymph node shows strong nucleolar and moderate nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680] - Staining of human stomach shows strong nucleolar and moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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NOP58 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQKVGDRKNYASAKLSELLPEEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMMAIAPNVTVMVGELVGARLIAHAGSLL
Marker
Nucleolar Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOP58
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NOP58 Protein (NBP1-81680PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NOP58 Antibody

  • HSPC120
  • NOL5
  • NOP5/NOP58
  • NOP58 ribonucleoprotein homolog (yeast)
  • NOP5nucleolar protein 58
  • Nucleolar protein 5
  • nucleolar protein NOP5/NOP58

Background

Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NOP58 Antibody (NBP1-81680) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NOP58 Antibody (NBP1-81680) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on NOP58

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Bioinformatics

Gene Symbol NOP58