![Western Blot: Coagulation Factor X Antibody [NBP1-33320] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Coagulation-Factor-X-Antibody-Western-Blot-NBP1-33320-img0013.jpg "Western Blot: Coagulation Factor X Antibody [NBP1-33320] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Factor X antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Coagulation Factor X. The exact sequence is proprietary. |
| Localization | Secreted |
| Predicted Species | Rhesus Macaque (95%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | F10 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | F10 |
![Immunocytochemistry/Immunofluorescence: Coagulation Factor X Antibody [NBP1-33320] - Methanol-fixed HeLa, using Factor X antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Coagulation-Factor-X-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33320-img0008.jpg "Immunocytochemistry/Immunofluorescence: Coagulation Factor X Antibody [NBP1-33320] - Methanol-fixed HeLa, using Factor X antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Coagulation Factor X Antibody [NBP1-33320] - NCI-N87 xenograft, using Factor X antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Coagulation-Factor-X-Antibody-Immunohistochemistry-Paraffin-NBP1-33320-img0009.jpg "Immunohistochemistry-Paraffin: Coagulation Factor X Antibody [NBP1-33320] - NCI-N87 xenograft, using Factor X antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Coagulation Factor X Antibody [NBP1-33320] - A. 30ug 293T whole cell lysate/extract, B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells 10 % SDS-PAGE gel, antibody dilution 1:5000.](http://images.novusbio.com/fullsize/Coagulation-Factor-X-Antibody-Western-Blot-NBP1-33320-img0010.jpg "Western Blot: Coagulation Factor X Antibody [NBP1-33320] - A. 30ug 293T whole cell lysate/extract, B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells 10 % SDS-PAGE gel, antibody dilution 1:5000.")
![Western Blot: Coagulation Factor X Antibody [NBP1-33320] - A. 30 ug GL261 whole lysate/extract, B. 30 ug C8D30 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Coagulation-Factor-X-Antibody-Western-Blot-NBP1-33320-img0011.jpg "Western Blot: Coagulation Factor X Antibody [NBP1-33320] - A. 30 ug GL261 whole lysate/extract, B. 30 ug C8D30 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.")
![N/A Coagulation Factor III/Tissue Factor [HRP]](https://images.novusbio.com/images2/DATA_Coagulation_Factor_III_DCF300_ELISA_625.jpg)
![N/A Coagulation Factor III/Tissue Factor [HRP]](https://images.novusbio.com/images2/Coagulation_Factor_III_DCF300_ELISA_61.jpg)
![Serpin C1/Antithrombin-III Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Serpin C1/Antithrombin-III Antibody [Unconjugated]")
![N/A TFPI [HRP]](https://images.novusbio.com/images2/DATA_TFPI_DTFP10_ELISA_841.jpg)
![N/A TFPI [HRP]](https://images.novusbio.com/images2/TFPI_DTFP10_ELISA_190.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0001.jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0003.jpg)
![Flow Cytometry Annexin V Apoptosis Kit [FITC]](https://images.novusbio.com/images/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0004.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")