Home » Neutrophil Elastase/ELA2 » Neutrophil Elastase/ELA2 Antibodies » Neutrophil Elastase/ELA2 Antibody (887105) [Unconjugated]

Neutrophil Elastase/ELA2 Antibody (887105) [Unconjugated]

 (5 Publications)
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Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB4517) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB4517) followed by HRP-conjugated Anti-Rat IgG ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
887105
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB4517-SP
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Neutrophil Elastase/ELA2 Antibody (887105) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neutrophil Elastase/ELA2
Met1-Arg260
Accession # Q3UP87
Specificity
Detects mouse Neutrophil Elastase/ELA2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ELANE
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB4517 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neutrophil Elastase/ELA2 Antibody (887105) [Unconjugated]

  • Bone marrow serine protease
  • EC 3.4.21
  • EC 3.4.21.37
  • ELA2
  • ELA2granulocyte-derived elastase
  • ELANE
  • elastase 2, neutrophil
  • elastase, neutrophil expressed
  • Elastase-2
  • GE
  • HLEelastase-2
  • HNE
  • Human leukocyte elastase
  • Leukocyte Elastase
  • Medullasin
  • NE
  • Neutrophil Elastase
  • PMN elastase
  • PMN-E
  • polymorphonuclear elastase
  • SCN1

Background

Neutrophil Elastase (ELA2), also known as Leukocyte Elastase, Bone marrow serine protease, Medullasin and ELANE, is a 25-30 kDa serine protease belonging to the peptidase S1 family. ELA2 is secreted by neutrophils during inflammation, it destroys bacteria and host tissue. ELA2 function is the degradation of many extracellular matrix macromolecules. ELA2 is localized to keratohyalin granules, where it was found to directly participate in pro-filaggrin processing.  When expressed aberrantly, ELA2 can cause emphysema or emphysematous changes, it involves breakdown of the lung structure and increased airspaces. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neutrophil Elastase/ELA2 Antibody (MAB4517)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(1)
Western Blot
(3)
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Mouse
(4)
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Showing Publications 1 - 5 of 5.
Publications using MAB4517 Applications Species
Ostermann, L;Seeliger, B;David, S;Flasche, C;Maus, R;Reinboth, MS;Christmann, M;Neumann, K;Brand, K;Seltmann, S;Bühling, F;Paton, JC;Roth, J;Vogl, T;Viemann, D;Welte, T;Maus, UA; S100A9 is indispensable for survival of pneumococcal pneumonia in mice PLoS pathogens 2023-07-01 [PMID: 37467233] (Western Blot, Mouse) Western Blot Mouse
D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice Cellular and Molecular Gastroenterology and Hepatology, 2022-06-02;0(0):. 2022-06-02 [PMID: 35660025] (ICC, IHC, Western Blot, Mouse) ICC, IHC, Western Blot Mouse
C Margaroli, MC Madison, L Viera, DW Russell, A Gaggar, KR Genschmer, JE Blalock A novel in vivo model for extracellular vesicle-induced emphysema JCI Insight, 2022-01-25;0(0):. 2022-01-25 [PMID: 35077395] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Ludwig T. Weckbach, Ulrich Grabmaier, Andreas Uhl, Sebastian Gess, Felicitas Boehm, Annette Zehrer et al. Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis Journal of Experimental Medicine 2/4/2019 [PMID: 30647120]
S Pires, D Parker IL-1? activation in response to Staphylococcus aureus lung infection requires inflammasome-dependent and independent mechanisms Eur. J. Immunol., 2018-08-19;0(0):. 2018-08-19 [PMID: 30051912] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol ELANE
Uniprot