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NEIL2 Antibody

Images

 
Western Blot: NEIL2 Antibody [NBP3-13112] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NEIL2 antibody (NBP3-13112) diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: NEIL2 Antibody [NBP3-13112] - NEIL2 antibody detects NEIL2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in ice-cold MeOH for 5 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NEIL2 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the center region of human NEIL2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEIL2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for NEIL2 Antibody

  • DNA glycosylase/AP lyase Neil2
  • EC 3.2.2.-
  • EC 4.2.99.18
  • endonuclease 8-like 2
  • Endonuclease VIII-like 2
  • FLJ31644
  • MGC2832
  • MGC4505
  • NEH2DNA-(apurinic or apyrimidinic site) lyase Neil2
  • nei endonuclease VIII-like 2 (E. coli)
  • Nei homolog 2
  • nei like 2 (E. coli)
  • nei like 2
  • NEI2
  • Nei-like protein 2

Background

NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226])[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEIL2