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NEIL1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: NEIL1 Antibody [NBP2-47358] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human lymphoid tissues shows strong nuclear positivity in germinal center cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NEIL1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEIL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NEIL1 Protein (NBP2-47358PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NEIL1 Antibody

  • DNA-(apurinic or apyrimidinic site) lyase Neil1
  • EC 3.2.2.-
  • EC 4.2.99.18
  • endonuclease 8-like 1
  • endonuclease VIII
  • Endonuclease VIII-like 1
  • FLJ22402
  • FPG1DNA glycosylase/AP lyase Neil1
  • hFPG1
  • NEH1
  • nei endonuclease VIII-like 1 (E. coli)
  • Nei homolog 1
  • NEI1
  • Nei-like protein 1

Background

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEIL1