MST1/STK4 Antibody - BSA Free

Images

 
Genetic Strategies: Western Blot: STK4 Antibody [NBP1-82865] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: STK4 Antibody [NBP1-82865] - Staining of human cell line CACO-2 shows localization to nucleoplasm, nuclear bodies & cytosol.
Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-82865] - Staining of human lymph node shows strong cytoplasmic positivity in cells outside reaction centra.
Western Blot: STK4 Antibody [NBP1-82865] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

MST1/STK4 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFEQKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STK4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
STK4 Protein (NBP1-82865PEP)
Publications
Read Publication using NBP1-82865.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MST1/STK4 Antibody - BSA Free

  • kinase responsive to stress 2
  • KRS2
  • Mammalian STE20-like protein kinase 1
  • mammalian sterile 20-like 1
  • MST1
  • serine/threonine-protein kinase 4
  • Serine/threonine-protein kinase Krs-2
  • STE20-like kinase MST1
  • STK4
  • YSK3

Background

STK4 is encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STK4 Antibody (NBP1-82865)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STK4 Antibody (NBP1-82865) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK4