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MCAM/CD146 Antibody [Unconjugated]

 (8 Publications)
 
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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MCAM/CD146 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) ...read more
MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 10 µg/mL for 3 hours at room temperature. ...read more
MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 15 µg/mL overnight at 4 ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for MCAM/CD146 at approximately 152 kDa (as indicated) using 10 µg/mL of ...read more
MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 5 µg/mL overnight at 4 ...read more
MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF932) at 3 µg/mL for 1 hour at ...read more
MCAM and COLEC12 are novel BEC- and LEC- specific markers.Two new endothelial markers MCAM and COLEC12 (selected from our analysis of Dataset B) were used to stain (A) normal human lymph nodes together with two ...read more
MCAM and COLEC12 are novel BEC- and LEC- specific markers.Two new endothelial markers MCAM and COLEC12 (selected from our analysis of Dataset B) were used to stain (A) normal human lymph nodes together with two ...read more
MCAM and COLEC12 are novel BEC- and LEC- specific markers.Two new endothelial markers MCAM and COLEC12 (selected from our analysis of Dataset B) were used to stain (A) normal human lymph nodes together with two ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF932
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF932-SP
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MCAM/CD146 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146
Met1-Gly559
Accession # AAA20824
Specificity
Detects human MCAM/CD46 in Western blots and direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhNCAM-L1, rhTROP-2 and recombinant mouse MCAM is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MCAM
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF932 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCAM/CD146 Antibody [Unconjugated]

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Background

MCAM, also known as MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAM/CD146 Antibody (AF932)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry.


Filter By Application
ICC
(1)
IHC
(2)
IHC-Fr
(2)
IHC-P
(1)
Immunohistochemistry
(1)
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Human
(6)
Mouse
(1)
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Showing Publications 1 - 8 of 8.
Publications using AF932 Applications Species
Dobersalske, C;Rauschenbach, L;Hua, Y;Berliner, C;Steinbach, A;Grüneboom, A;Kokkaliaris, KD;Heiland, DH;Berger, P;Langer, S;Tan, CL;Stenzel, M;Landolsi, S;Weber, F;Darkwah Oppong, M;Werner, RA;Gull, H;Schröder, T;Linsenmann, T;Buck, AK;Gunzer, M;Stuschke, M;Keyvani, K;Forsting, M;Glas, M;Kipnis, J;Steindler, DA;Reinhardt, HC;Green, EW;Platten, M;Tasdogan, A;Herrmann, K;Rambow, F;Cima, I;Sure, U;Scheffler, B; Cranioencephalic functional lymphoid units in glioblastoma Nature medicine 2024-07-31 [PMID: 39085419] (Immunohistochemistry, Human) Immunohistochemistry Human
Runhua Feng, Yuling Wang, Vijaya Ramachandran, Qinhong Ma, Matthew M. May, Ming Li et al. Characterization of novel neutralizing mouse monoclonal antibody JM1-24-3 developed against MUC18 in metastatic melanoma Journal of Experimental & Clinical Cancer Research 2020-12-05 [PMID: 33278894]
EJ Kim, SG Seo, HS Shin, DJ Lee, JH Kim, DY Lee Platelet-Derived Growth Factor Receptor-Positive Pericytic Cells of White Adipose Tissue from Critical Limb Ischemia Patients Display Mesenchymal Stem Cell-Like Properties Clin Orthop Surg, 2017-05-08;9(2):239-248. 2017-05-08 [PMID: 28567229] (IHC, Human) IHC Human
Zhang P, He D, Chen Z, Pan Q, Du F, Zang X, Wang Y, Tang C, Li H, Lu H, Yao X, Jin J, Ma X Chemotherapy enhances tumor vascularization via Notch signaling-mediated formation of tumor-derived endothelium in breast cancer. Biochem Pharmacol, 2016-08-09;118(0):18-30. 2016-08-09 [PMID: 27520484] (ICC, Human) ICC Human
Gu , Zirong, Imai , Fumiyasu, Kim , In Jung, Fujita , Hiroko, Katayama , Kei ichi, Mori , Kensaku, Yoshihara , Yoshihir, Yoshida , Yutaka Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion. PLoS ONE, 2015-03-31;10(3):e0121550. 2015-03-31 [PMID: 25826454] (IHC, Mouse) IHC Mouse
Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients. PLoS ONE, 2015-03-24;10(3):e0121421. 2015-03-24 [PMID: 25803686] (IHC-P, Human) IHC-P Human
Keuschnigg , Johannes, Karinen , Sirkku, Auvinen , Kaisa, Irjala , Heikki, Mpindi , John-Pat, Kallioniemi , Olli, Hautaniemi , Sampsa, Jalkanen , Sirpa, Salmi , Marko Plasticity of blood- and lymphatic endothelial cells and marker identification. PLoS ONE, 2013-09-10;8(9):e74293. 2013-09-10 [PMID: 24058540] (IHC-Fr, Human) IHC-Fr Human
Rossi E, Sanz-Rodriguez F, Eleno N, Duwell A, Blanco F, Langa C, Botella L, Cabanas C, Lopez-Novoa J, Bernabeu C Endothelial endoglin is involved in inflammation: role in leukocyte adhesion and transmigration. Blood, 2012-10-16;121(2):403-15. 2012-10-16 [PMID: 23074273] (IHC-Fr, Human) IHC-Fr Human

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Bioinformatics

Gene Symbol MCAM
Uniprot