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MCAM/CD146 Antibody [Biotin]

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MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF932) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF932) at 15 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF932
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF932
Carrier Free
AF932
Carrier Free
AF932-SP
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MCAM/CD146 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146
Met1-Gly559
Accession # AAA20824
Specificity
Detects human MCAM/CD146 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) ALCAM, recombinant mouse (rm) MAdCAM-1, and rmOCAM is observed and less than 1% cross-reactivity with rhBCAM, rhEpCAM, rhNCAM-L1, and rhTROP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MCAM
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System
Publications
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BAF932 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCAM/CD146 Antibody [Biotin]

  • CD146 antigen
  • CD146
  • cell surface glycoprotein MUC18
  • Cell surface glycoprotein P1H12
  • L-Gicerin
  • MCAM
  • melanoma adhesion molecule
  • melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
  • Melanoma-associated antigen A32
  • Melanoma-associated antigen MUC18
  • MUC18
  • MUC18Gicerin

Background

MCAM, also known as MUC18, is an Ig-superfamily glycoprotein that shows significant homology to several cell adhesion molecules, notably N-CAM. It was identified as an antigen on metastatic melanoma cells and is also expressed on a variety of normal and transformed cell types. MCAM expression is associated with tumor progression and metastasis, and may be involved in embryonic neuronal development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAM/CD146 Antibody (BAF932)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Luminex Development.


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Bioinformatics

Gene Symbol MCAM
Uniprot