![Western Blot: MBD1 Antibody [NBP2-59293] - Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/MBD1-Antibody-Western-Blot-NBP2-59293-img0001.jpg "Western Blot: MBD1 Antibody [NBP2-59293] - Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | The exact immunogen is propietary information. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MBD1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide and 0.05% ProClin 300 |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MBD1 Antibody (NBP2-59293)Find related products by research area.
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| Gene Symbol | MBD1 |
![Chromatin Immunoprecipitation: MBD1 Antibody [NBP2-59293] - ChIP assays were performed using human osteosarcoma (U2OS) cells, the antibody against MBD1 and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 1.5 ug of antibody were used per ChIP experiment. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/MBD1-Antibody-Chromatin-Immunoprecipitation-NBP2-59293-img0003.jpg "Chromatin Immunoprecipitation: MBD1 Antibody [NBP2-59293] - ChIP assays were performed using human osteosarcoma (U2OS) cells, the antibody against MBD1 and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 1.5 ug of antibody were used per ChIP experiment. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![ELISA: MBD1 Antibody [NBP2-59293] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1, crude serum and Flow Through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.](http://images.novusbio.com/fullsize/MBD1-Antibody-ELISA-NBP2-59293-img0002.jpg "ELISA: MBD1 Antibody [NBP2-59293] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1, crude serum and Flow Through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")