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MBD3 Antibody (9X1J7)

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Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunohistochemistry-Paraffin: MBD3 Antibody (9X1J7) [NBP3-16718] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MBD3 Rabbit mAb (NBP3-16718) at dilution of 1:100 (40x lens).Perform microwave ...read more
Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
9X1J7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MBD3 Antibody (9X1J7) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MBD3 (O95983). MERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALP
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MBD3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MBD3 Antibody (9X1J7)

  • MBD3
  • methyl-CpG binding domain protein 3
  • methyl-CpG-binding domain protein 3
  • Methyl-CpG-binding protein MBD3

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional MBD3 Products

Array NBP3-16718

Research Areas for MBD3 Antibody (NBP3-16718)

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Blogs on MBD3

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Bioinformatics

Gene Symbol MBD3