![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunocytochemistry-Immunofluorescence-NBP1-41201-img0014.jpg "Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clone | 13D10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | The complete coding region (amino acids 1-425) of RBBP4/RbAp48 expressed in E. coli. |
| Localization | Nuclear |
| Specificity | This antibody will recognize human RbAp48. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RBBP4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Fugui Li |
WB | Human | 09/23/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for RBBP4/RbAp48 Antibody (NBP1-41201)Find related products by research area.
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![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Western-Blot-NBP1-41201-img0012.jpg "Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract")
![Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunoprecipitation-NBP1-41201-img0017.jpg "Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-western-blot-196202494114..jpg "Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
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