MBD1 Antibodies
We offer MBD1 Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each MBD1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MBD1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the MBD1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MBD1 polyclonal antibodies and browse our MBD1 monoclonal antibody catalog.
Alternate Names for MBD1 Antibodies
anti-MBD1 antibody, anti-CXXC-type zinc finger protein 3 antibody, anti-methyl-CpG binding domain protein 1 antibody, anti-methyl-CpG binding domain protein 1 isoform PCM1 antibody, anti-methyl-CpG-binding domain protein 1 antibody, anti-Methyl-CpG-binding protein MBD1 antibody, anti-PCM1CXXC3RFT antibody, anti-Protein containing methyl-CpG-binding domain 1 antibody, anti-the regulator of fibroblast growth factor 2 (FGF-2) transcription antibody
11 Results for "mbd1" in Primary Antibodies
![Knockdown Validated: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NB100-56537) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Knockdown-Validated-NB100-56537-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NB100-56537) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis using Azide and BSA Free version of NB100-56537. Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Western-Blot-NB100-56537-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - NIH3T3 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunocytochemistry-Immunofluorescence-NB100-56537-img0007.jpg)
![Immunohistochemistry-Paraffin: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of MBD1 (100B272.1) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and nuclear staining was observed.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunohistochemistry-Paraffin-NB100-56537-img0008.jpg)
![Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Western-Blot-NB100-56537-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunocytochemistry-Immunofluorescence-NB100-56537-img0006.jpg)
![Knockdown Validated: MBD1 Antibody [NB100-56330] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human MBD1 Polyclonal Antibody (Catalog # NB100-56330) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/MBD1-Antibody-Knockdown-Validated-NB100-56330-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: MBD1 Antibody [NB100-56330] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human MBD1 Polyclonal Antibody (Catalog # NB100-56330) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: MBD1 Antibody [NB100-56330] - Analysis of MBD1 in HeLa cell lysate with this antibody. A protein band with an approximate molecular weight of 55 kDa was detected.](http://images.novusbio.com/fullsize/MBD1-Antibody-Western-Blot-NB100-56330-img0002.jpg)
![Western Blot: MBD1 Antibody (PSH01-79) [NBP3-32571] - Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (NBP3-32571) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C2C12 cell lysate Lane 4: Mouse kidney tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse lung tissue lysate Lane 7: Mouse spleen tissue lysate Lane 8: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32571) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32571_rabbit-mbd1-mab-psh01-79-278202415242646.jpg "Western Blot: MBD1 Antibody (PSH01-79) [NBP3-32571] - Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (NBP3-32571) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C2C12 cell lysate Lane 4: Mouse kidney tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse lung tissue lysate Lane 7: Mouse spleen tissue lysate Lane 8: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32571) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: MBD1 Antibody (PSH01-79) [NBP3-32571] - Immunocytochemistry analysis of NIH/3T3 cells labeling MBD1 with Rabbit anti-MBD1 antibody (NBP3-32571) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MBD1 antibody (NBP3-32571) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32571_rabbit-mbd1-mab-psh01-79-29202410484860.jpg)
![Flow Cytometry: MBD1 Antibody (PSH01-79) [NBP3-32571] - Flow cytometric analysis of NIH/3T3 cells labeling MBD1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32571, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32571_rabbit-mbd1-mab-psh01-79-29202416453212.jpg)
![Western Blot: MBD1 Antibody [NBP2-59293] - Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/MBD1-Antibody-Western-Blot-NBP2-59293-img0001.jpg "Western Blot: MBD1 Antibody [NBP2-59293] - Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: MBD1 Antibody [NBP2-59293] - ChIP assays were performed using human osteosarcoma (U2OS) cells, the antibody against MBD1 and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 1.5 ug of antibody were used per ChIP experiment. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/MBD1-Antibody-Chromatin-Immunoprecipitation-NBP2-59293-img0003.jpg)
![ELISA: MBD1 Antibody [NBP2-59293] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1, crude serum and Flow Through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.](http://images.novusbio.com/fullsize/MBD1-Antibody-ELISA-NBP2-59293-img0002.jpg)
![Western Blot: MBD1 Antibody [NBP2-68876] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/MBD1-Antibody-Western-Blot-NBP2-68876-img0001.jpg "Western Blot: MBD1 Antibody [NBP2-68876] - Analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody [NBP2-68876] - Staining of human cell line HaCaT shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/MBD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68876-img0002.jpg)
![Western Blot: MBD1 Antibody (SR2117) [NBP3-21764] - Western blot analysis using NBP3-21764 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21764_rabbit-mbd1-mab-sr2117-5720231523492.jpg "Western Blot: MBD1 Antibody (SR2117) [NBP3-21764] - Western blot analysis using NBP3-21764 in HeLa cell lysate.")
![Western Blot: MBD1 Antibody [NBP3-18674] - Figure 2. Western blot analysis using the antibody directed against MBD1 HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody against MBD1 diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/MBD1-Antibody-Western-Blot-NBP3-18674-img0001.jpg "Western Blot: MBD1 Antibody [NBP3-18674] - Figure 2. Western blot analysis using the antibody directed against MBD1 HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody against MBD1 diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.")
![ELISA: MBD1 Antibody [NBP3-18674] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1 in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.](http://images.novusbio.com/fullsize/MBD1 Antibody-ELISA-NBP3-18674-img0002.jpg)
![Western Blot: MBD1 Antibody (PSH01-78) [NBP3-32572] - Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (NBP3-32572) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Human kidney tissue lysate Lane 6: Mouse kidney tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Mouse lung tissue lysate Lane 10: Rat brain tissue lysate Lane 11: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32572) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32572_rabbit-mbd1-mab-psh01-78-27820241631621.jpg "Western Blot: MBD1 Antibody (PSH01-78) [NBP3-32572] - Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (NBP3-32572) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Human kidney tissue lysate Lane 6: Mouse kidney tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Mouse testis tissue lysate Lane 9: Mouse lung tissue lysate Lane 10: Rat brain tissue lysate Lane 11: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32572) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody (10B10) [NBP3-26147] - Staining of MCF-7 cell with MBD1 Antibody (10B10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 498-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26147_rabbit-mbd1-mab-10b10-28220241312230.jpg "Immunocytochemistry/Immunofluorescence: MBD1 Antibody (10B10) [NBP3-26147] - Staining of MCF-7 cell with MBD1 Antibody (10B10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 498-conjugated Goat Anti-Rabbit IgG (H+L).")
![Immunohistochemistry-Paraffin: MBD1 Antibody (CPTC-MBD1-1) [NBP3-07275] - Formalin-fixed, paraffin-embedded human colon stained with MBD1 Mouse Monoclonal Antibody (CPTC-MBD1-1)](http://images.novusbio.com/fullsize/MBD1-Antibody-CPTC-MBD1-1-Immunohistochemistry-Paraffin-NBP3-07275-img0001.jpg "Immunohistochemistry-Paraffin: MBD1 Antibody (CPTC-MBD1-1) [NBP3-07275] - Formalin-fixed, paraffin-embedded human colon stained with MBD1 Mouse Monoclonal Antibody (CPTC-MBD1-1)")