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LRPPRC Antibody

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Western Blot: LRPPRC Antibody [NBP2-19373] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRPPRC antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Genetic Strategies: Western Blot: LRPPRC Antibody [NBP2-19373] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRPPRC ...read more
Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-19373] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Western Blot: LRPPRC Antibody [NBP2-19373] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: LRPPRC Antibody [NBP2-19373] - LRPPRC antibody [N2N3] detects LRPPRC protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

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LRPPRC Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human LRPPRC. The exact sequence is proprietary.
Localization
Mitochondrion; Nucleus; nucleoplasm; Nucleus inner membrane; Nucleus outer membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LRPPRC
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
158 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for LRPPRC Antibody

  • CLONE-23970
  • leucine-rich PPR-motif containing
  • mitochondrial leucine-rich PPR motif-containing protein
  • mitochondrial

Background

This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. Transcripts ranging in size from 4.8 to 7.0 kb which result from alternative polyadenylation have been reported for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LRPPRC
Uniprot