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Jak3 Antibody (452506)

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Western blot shows lysates of TF-1 human erythroleukemic cell line. PVDF membrane was probed with 2 µg/mL of Human Jak3 Monoclonal Antibody (Catalog # MAB4699) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jak3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of TF-1 human erythroleukemic cell line. PVDF membrane was probed with 2 µg/mL of Human Jak3 Monoclonal Antibody (Catalog # MAB4699) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
452506
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4699-SP
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Jak3 Antibody (452506) Summary

Immunogen
E. coli-derived recombinant human Jak3
Gly46-Thr209
Accession # P52333
Specificity
Detects human Jak3 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
JAK3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jak3 Antibody (452506)

  • EC 2.7.10
  • Jak3
  • JAK3_HUMAN
  • JAK-3EC 2.7.10.2
  • JAKL
  • JAKLtyrosine-protein kinase JAK3
  • Janus kinase 3Leukocyte janus kinase
  • LJAK
  • L-JAKJanus kinase 3 (a protein tyrosine kinase, leukocyte)

Background

Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Also known as Leukocyte Janus Kinase (LJak), Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically the IL-2 and IL-4 receptors. Activation of Jak3 is associated with the rapid tyrosine phosphorylation of STAT proteins. Defects in Jak3 cause an autosomal severe combined immunodeficiency disease (SCID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Jak3 Antibody
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Jak3 Antibody (452506)
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol JAK3
Uniprot