![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Quantification of knockdown of Beta1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine Beta1 integrin expression and spatial distribution of Beta1 integrin 46 h post-transfection. Image analysis software was used to quantify Beta1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in Beta1 integrin expression at microstructure surfaces was quantified. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0005.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Quantification of knockdown of Beta1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine Beta1 integrin expression and spatial distribution of Beta1 integrin 46 h post-transfection. Image analysis software was used to quantify Beta1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in Beta1 integrin expression at microstructure surfaces was quantified. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fcell.2019.00224/full) licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, IHC, KD |
| Clone | 12G10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract |
| Epitope | Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain. |
| Localization | Type 1 membrane protein |
| Marker | Stem Cell Marker |
| Specificity | NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ITGB1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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reviewed by:
Verified Customer |
ICC | Human | 11/29/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Integrin beta 1/CD29 Antibody (NB100-63255)Find related products by research area.
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Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ... Read full blog post. |
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![Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-12G10-Flow-Cytometry-NB100-63255-img0003.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0004.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels. Image from verified customer review.")
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