Home » Integrin beta 1/CD29 » Integrin beta 1/CD29 Antibodies » Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1]
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Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clone
12G10
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-63255AF350
Alexa Fluor 405
NB100-63255AF405
Alexa Fluor 488
NB100-63255AF488
Alexa Fluor 532
NB100-63255AF532
Alexa Fluor 594
NB100-63255AF594
Alexa Fluor 647
NB100-63255AF647
Alexa Fluor 700
NB100-63255AF700
Alexa Fluor 750
NB100-63255AF750
Allophycocyanin
NB100-63255APC
Allophycocyanin/Cy7
NB100-63255APCCY7
Biotin
NB100-63255B
DyLight 350
NB100-63255UV
DyLight 405
NB100-63255V
DyLight 488
NB100-63255G
DyLight 550
NB100-63255R
DyLight 594
NB100-63255DL594
DyLight 650
NB100-63255C
DyLight 680
NB100-63255FR
DyLight 755
NB100-63255IR
FITC
NB100-63255F
HRP
NB100-63255H
Janelia Fluor 525
NB100-63255JF525
Janelia Fluor 549
NB100-63255JF549
Janelia Fluor 646
NB100-63255JF646
Janelia Fluor 669
NB100-63255JF669
PE
NB100-63255PE
PE/Atto594
NB100-63255PEATT594
PE/Cy5.5
NB100-63255PECY55
PE/Cy7
NB100-63255PECY7
PerCP
NB100-63255PCP
Unconjugated
NB100-63255
mFluor Violet 450 SE
NB100-63255MFV450
mFluor Violet 500 SE
NB100-63255MFV500
mFluor Violet 610 SE
NB100-63255MFV610
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Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract
Epitope
Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.
Localization
Type 1 membrane protein
Marker
Stem Cell Marker
Specificity
NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted cross-reactivities: Mink, Rabbit

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Integrin beta 1/CD29 Antibody (12G10) [CoraFluor™ 1]

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NB100-63255CL1). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Secondary Antibodies

 

Isotype Controls

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Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ...  Read full blog post.
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Bioinformatics

Gene Symbol ITGB1