![Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Western blot analysis of lysates from wild type(WT) and IMP Dehydrogenase 2/IMPDH2 knockout (KO) NIH/3T3(KO) cells, using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16786_rabbit-imp-dehydrogenase-2-impdh2-mab-4x3s10-82202520989.jpg "Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Western blot analysis of lysates from wild type(WT) and IMP Dehydrogenase 2/IMPDH2 knockout (KO) NIH/3T3(KO) cells, using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 4X3S10 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human IMP Dehydrogenase 2/IMPDH2 (P12268). QANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVI |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | IMPDH2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot IMPDH1 Antibody [Unconjugated]](https://images.novusbio.com/images2/IMP_Dehydrogenase_1_AF8554_Western_Blot_16658.jpg)
![Immunocytochemistry IMPDH1 Antibody [Unconjugated]](https://images.novusbio.com/images2/IMP_Dehydrogenase_1_AF8554_Immunocytochemistry__Immunofluorescence_17605.jpg)
![Immunocytochemistry IMPDH1 Antibody [Unconjugated]](https://images.novusbio.com/images2/af8554_human-mouse-rat-imp-dehydrogenase-1-impdh1-aff-pur-pab-immunocytochemistry-842022162057.jpg)
![Intracellular Staining by Flow Cytometry Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-1_AF1152_Flow_Cytometry_11328.jpg)
![Simple Western Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin1_AF1152_Simple_Western_16673.jpg)
![Immunohistochemistry Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-1_AF1152_Immunohistochemistry_6684.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for IMP Dehydrogenase 2/IMPDH2 Antibody (NBP3-16786)Find related products by research area.
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| Gene Symbol | IMPDH2 |
![Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C6 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16786_rabbit-imp-dehydrogenase-2-impdh2-mab-4x3s10-522025113894.jpg%3Ft=1704262370 "Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C6 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C6 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16786_rabbit-imp-dehydrogenase-2-impdh2-mab-4x3s10-82202520104010.jpg "Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C6 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of NIH/3T3 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16786_rabbit-imp-dehydrogenase-2-impdh2-mab-4x3s10-8220252010405.jpg "Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of NIH/3T3 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C2C12 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16786_rabbit-imp-dehydrogenase-2-impdh2-mab-4x3s10-8220252010402.jpg "Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C2C12 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Western blot analysis of various lysates, using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-16786_rabbit-imp-dehydrogenase-2-impdh2-mab-4x3s10-8220252010436.jpg "Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Western blot analysis of various lysates, using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")