Home » IL-7R alpha/CD127 » IL-7R alpha/CD127 Antibodies » IL-7R alpha/CD127 Antibody [Unconjugated]
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IL-7R alpha/CD127 Antibody [Unconjugated]

 (7 Publications)
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IL-7 response of CD4+ T cells from tuberculosis patients and healthy contacts.(a) IL-7-induced (10 ng/ml) STAT5 phosphorylation of CD4+ T cells from tuberculosis patients (n = 22) and healthy contacts (n = 24) measured by flow cytometry. The STAT5 phosphorylation level of non-stimulated cells has been subtracted of all values. (b) IFN gamma /CD40L-expressing CD4+ T cells after PPD re-stimulation in the presence or absence of IL-7 detected by flow cytometry. Non-stimulated values with or without IL-7 have been subtracted. (c) Induction of IFN gamma /CD40L-expressing CD4+ T cells by IL-7 and PPD stimulation. Absolute differences as compared to PPD alone are shown. (d) Plasma IL-6 levels of tuberculosis patients (n = 20) and healthy contacts (n = 24). An arbitrary threshold indicated by a dotted line was set to define tuberculosis patients with high (IL-6high) and low (IL-6low) concentrations of plasma IL-6. (e) STAT5 phosphorylation, and IL-7-induced PPD response (f) for tuberculosis patients with high or low plasma IL-6 level, as defined in (d). Median and interquartile range is depicted, and exact Mann-Whitney U test was applied for comparison of groups, whereas paired data was evaluated by Wilcoxon Signed-Rank test. Nominal p-values are indicated as * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.ppat.1006425), licensed under a CC-BY license. Not internally tested by R&D Systems.
IL-7 response of CD4+ T cells from tuberculosis patients and healthy contacts.(a) IL-7-induced (10 ng/ml) STAT5 phosphorylation of CD4+ T cells from tuberculosis patients (n = 22) and healthy contacts (n = 24) measured ...read more
Membrane-associated IL-7R expression on CD4+ and CD8+ T cells.Membrane-associated IL-7R expression of CD4+ (left graphs) and CD8+ (right graphs) T cells from tuberculosis patients (n = 17) and healthy contacts (n = 21) ...read more
Membrane-associated IL-7R expression on CD4+ and CD8+ T cells.Membrane-associated IL-7R expression of CD4+ (left graphs) and CD8+ (right graphs) T cells from tuberculosis patients (n = 17) and healthy contacts (n = 21) ...read more
Membrane-associated IL-7R expression on CD4+ and CD8+ T cells.Membrane-associated IL-7R expression of CD4+ (left graphs) and CD8+ (right graphs) T cells from tuberculosis patients (n = 17) and healthy contacts (n = 21) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF306
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-306-SP
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IL-7R alpha/CD127 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha /CD127
Specificity
Detects human IL-7 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant rat IL-7 R alpha and less than 5% cross-reactivity with recombinant mouse IL‑7 R alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL7R
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF-306-PB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-7R alpha/CD127 Antibody [Unconjugated]

  • CD127 antigen
  • CD127
  • CD127ILRA
  • CDW127
  • IL-7 R alpha
  • IL-7 receptor subunit alpha
  • IL7R alpha
  • IL-7R alpha
  • IL-7R subunit alpha
  • IL7R
  • IL7RA
  • IL-7Ra
  • IL-7R-alpha
  • interleukin 7 receptor alpha chain
  • interleukin 7 receptor isoform H5-6
  • interleukin 7 receptor
  • interleukin-7 receptor subunit alpha

Background

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha . IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

  1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
  2. Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
  3. Goodwin, R.G. et al. (1990) Cell 60:941.
  4. Noguchi, M. et al. (1993) Science 262:1877.
  5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
  8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
  10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
  15. Jahn, T. et al. (2007) Blood 110:1840.
  16. Lai, L. et al. (2006) Blood 107:1776.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-7R alpha/CD127 Antibody (AF-306-PB)(7)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 5 applications: Cell Selection, Flow Cytometry, Immunoprecipitation, Luminex Development, Western Blot.


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Cell Selection
(1)
Flow Cytometry
(3)
Immunoprecipitation
(1)
Luminex Development
(1)
Western Blot
(1)
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Human
(6)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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Showing Publications 1 - 7 of 7.
Publications using AF-306-PB Applications Species
JD Harelimana, HS Ahor, B Benner, S Hellmuth, E Adankwah, D Minadzi, W Aniagyei, MNK Lamptey, J Arthur, A Yeboah, MK Abass, LB Debrah, DO Owusu, E Mayatepek, J Seyfarth, RO Phillips, M Jacobsen Cytokine-induced transient monocyte IL-7Ra expression and the serum milieu in tuberculosis European Journal of Immunology, 2022-03-25;0(0):. 2022-03-25 [PMID: 35279828] (Human) Human
AK Steele, L Carrasco-M, DL Sodora, AM Crawley Increased soluble IL-7 receptor concentrations associate with improved IL-7 therapy outcomes in SIV-infected ART-treated Rhesus macaques PLoS ONE, 2017-12-19;12(12):e0188427. 2017-12-19 [PMID: 29261677] (Luminex Development, Primate - Macaca mulatta (Rhesus Macaque)) Luminex Development Primate - Macaca mulatta (Rhesus Macaque)
C Lundtoft, A Afum-Adjei, J Rimpler, K Harling, N Nausch, M Kohns, E Adankwah, F Lang, L Olbrich, E Mayatepek, E Owusu-Dabo, M Jacobsen Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis PLoS Pathog., 2017-06-05;13(6):e1006425. 2017-06-05 [PMID: 28582466] (Flow Cytometry, Human) Flow Cytometry Human
Shin M, You S, Kang Y, Lee N, Yoo S, Park K, Kang K, Kim S, Mohanty S, Shaw A, Montgomery R, Hwang D, Kang I DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Ralphalow and IL-7Ralphahigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine. J Immunol, 2015-08-14;195(6):2861-9. 2015-08-14 [PMID: 26276874] (Flow Cytometry, Human) Flow Cytometry Human
Takao T, Asanoma K, Kato K Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line. PLoS ONE, 2011-07-07;6(7):e21990. 2011-07-07 [PMID: 21760941] (Cell Selection, Human) Cell Selection Human
Faller EM, Sugden SM, McVey MJ Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation. J. Immunol., 2010-07-26;185(5):2854-66. 2010-07-26 [PMID: 20660706] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
Mengshol JA, Golden-Mason L, Arikawa T A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection. PLoS ONE, 2010-03-04;5(3):e9504. 2010-03-04 [PMID: 20209097] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol IL7R