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Ikaros/IKZF1 Antibody

 
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Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining in human tonsil and cerebral cortex tissues using anti-IKZF1 antibody. Corresponding IKZF1 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining in human tonsil and cerebral cortex tissues using anti-IKZF1 antibody. Corresponding IKZF1 RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human cerebral cortex, kidney, testis and tonsil using Anti-IKZF1 antibody NBP2-38242 (A) shows similar ...read more
Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human tonsil shows high expression.
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human kidney using Anti-IKZF1 antibody.
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human testis using Anti-IKZF1 antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

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Ikaros/IKZF1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DADEGQDMSQVSGKESPPVSDTPDEGDEPMPIPEDLSTTSGGQQSSKSDRVVASNVKVETQSDEENGRACEMNGEECAEDLRMLDASGEKMNGSH
Predicted Species
Mouse (94%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IKZF1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 -1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Simple Western reported by internal validation
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western internal validation: RPMI 8266 cell line at 1.0 mg/ml as sample; separated by size; antibody dilution of 1:50; observed molecular weight was 35-77 kDa; matrix was 12-230 kDa; detected by Chemiluminescence.
Control Peptide
Ikaros/IKZF1 Protein (NBP2-38242PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ikaros/IKZF1 Antibody

  • DNA-binding protein Ikaros
  • hIk-1
  • IK1
  • IK1LyF-1
  • Ikaros (zinc finger protein)
  • IKAROS family zinc finger 1 (Ikaros)
  • Ikaros family zinc finger protein 1
  • Ikaros
  • IKAROSLymphoid transcription factor LyF-1
  • IKZF1
  • LYF1
  • LyF-1
  • LYF1PRO0758
  • PRO0758
  • zinc finger protein, subfamily 1A, 1 (Ikaros)
  • ZNFN1A1
  • ZNFN1A1CLL-associated antigen KW-6

Background

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IKZF1
Uniprot