![Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Analysis of Histone H4 R3me1 in NIH/3T3 histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-Western-Blot-NB21-2011-img0002.jpg "Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Analysis of Histone H4 R3me1 in NIH/3T3 histone preps.")
| Reactivity | Hu, Mu, Rt, MaSpecies Glossary |
| Applications | WB, ICC/IF, mIF, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.43 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2011AF405 | |||
| Alexa Fluor 488 | NB21-2011AF488 | |||
| Alexa Fluor 647 | NB21-2011AF647 | |||
| Alexa Fluor 700 | NB21-2011AF700 | |||
| Biotin | NB21-2011B | |||
| DyLight 405 | NB21-2011V | |||
| DyLight 488 | NB21-2011G | |||
| DyLight 550 | NB21-2011R | |||
| DyLight 650 | NB21-2011C | |||
| FITC | NB21-2011F | |||
| HRP | NB21-2011H | |||
| Immunogen | Synthetic monomethylated peptide surrounding Arginine 3 of human Histone H4 [Swiss Prot P62805]. |
| Modification | Monomethyl Arg3 |
| Localization | Nucleus. Chromosome. |
| Marker | H4R3me1 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HIST4H4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot a band is seen ~13 kDa. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.43 mg/ml |
| Purity | Immunogen affinity purified |
![Histone H4 [Monomethyl Arg3] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Histone H4 [Monomethyl Arg3] Antibody")
Secondary Antibodies |
Isotype Controls |
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H4 - Monitoring global chromatin structure through histone modifications Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro... Read full blog post. |
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Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
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Histone H4: Implications in Liver Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
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"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Histone H4 R3me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2011-img0005.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Histone H4 R3me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-NB21-2011-img0006.jpg "Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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