![Western Blot: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Analysis of Histone H4 meIK20 in HeLa histone lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-5E10-D8-Azide-and-BSA-Free-Western-Blot-NBP2-80771-img0001.jpg "Western Blot: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Analysis of Histone H4 meIK20 in HeLa histone lysates. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Ce, Dr, Ma, YeSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, mIF, ChIP |
| Clone | 5E10-D8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805] |
| Localization | Nucleus |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HIST4H4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This Histone H4 [Monomethyl Lys20] (5E10-D8) antibody is useful in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is seen at ~11 kDa. |
| Theoretical MW | 11 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15 M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H4 Antibody (NBP2-80771)Find related products by research area.
|
|
H4 - Monitoring global chromatin structure through histone modifications Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro... Read full blog post. |
|
Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
|
Histone H4: Implications in Liver Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
|
"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HIST4H4 |
![Immunocytochemistry/Immunofluorescence: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-5E10-D8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80771-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue). Image from the standard format of this antibody.")
![Dot Blot: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-5E10-D8-Azide-and-BSA-Free-Dot-Blot-NBP2-80771-img0004.jpg "Dot Blot: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20. Image from the standard format of this antibody.")
![ELISA: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - HeLa extracts. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-5E10-D8-Azide-and-BSA-Free-ELISA-NBP2-80771-img0003.jpg "ELISA: Histone H4 Antibody (5E10-D8) - Azide and BSA Free [NBP2-80771] - HeLa extracts. Image from the standard format of this antibody.")
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
![Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/mab2676_human-mouse-rat-cbp-mab-clone-257003-immunocytochemistry-101220208237.jpg)
![Western Blot eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81898-img0005.jpg)
![Immunohistochemistry-Paraffin eIF4E [p Ser209] Antibody](https://images.novusbio.com/images/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")