![Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Chromatin-Immunoprecipitation-NBP1-30091-img0001.jpg "Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.")
| Reactivity | Hu, Mu, Rt, Ce, Dr, Ma, YeSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, ChIP |
| Clone | 5E10-D8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805] |
| Modification | Monomethyl Lys20 |
| Localization | Nucleus |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H4C16 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This Histone H4 [Monomethyl Lys20] (5E10-D8) antibody is useful in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is seen at ~11 kDa. |
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| Theoretical MW | 11 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine and 0.15M NaCl |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Other | 09/12/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H4 Antibody (NBP1-30091)Find related products by research area.
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Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
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Histone H4: Implications in Liver Cancer Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t... Read full blog post. |
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"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0009.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Western-Blot-NBP1-30091-img0008.jpg "Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0006.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).")
![Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Dot-Blot-NBP1-30091-img0002.jpg "Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20.")
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