![Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095] - Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC2 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59 kDa for HDAC2.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Western-Blot-NBP1-44095-img0003.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095] - Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC2 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59 kDa for HDAC2.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Histone Deacetylase 2/Histone Deacetylase 2/HDAC2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near C-terminal region of human Histone Deacetylase 2/HDAC2. (Uniprot: Q92769) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HDAC2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~59 kDa corresponding to the appropriate cell lysate or extract. |
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| Publications |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol |
| Preservative | No Preservative |
| Purity | Immunogen affinity purified |
| Publication using NBP1-44095 | Applications | Species |
|---|---|---|
| de Ruijter AJ et al. Histone deacetylases (HDACs): characterization of the classical HDAC family. Biochem J;370(Pt 3):737-49. 2003-03-15 [PMID: 12429021] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone Deacetylase 2/HDAC2 Antibody (NBP1-44095)Find related products by research area.
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