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GRF2 Antibody (OTI2F5) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

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GRF2 Antibody (OTI2F5) [Janelia Fluor® 669] Summary

Immunogen
Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAPGEF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for GRF2 Antibody (OTI2F5) [Janelia Fluor® 669]

  • C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
  • CRK SH3-binding GNRP
  • DKFZp781P1719
  • Guanine nucleotide-releasing factor 2
  • Protein C3G
  • Rap guanine nucleotide exchange factor (GEF) 1
  • rap guanine nucleotide exchange factor 1

Background

GRF2 encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRF2 Antibody (NBP2-70846JF669) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-70846JF669

Research Areas for GRF2 Antibody (NBP2-70846JF669)

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Calculators

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Bioinformatics

Gene Symbol RAPGEF1